Specifications Table

Product Name – ACES, 99%, extra pure
Quantity/Pack Size – 25GR, 100GR, 250GR
Form – Powder
Grade – Extra Pure
Application – Buffering agent in biochemical research, electrophoresis, cell culture

Product Overview

ACES (N-(2-Acetamido)-2-aminoethanesulfonic acid) is a high-purity biochemical buffer with a pKa of 6.8, making it ideal for maintaining stable pH in biological systems. This 99% extra pure grade ensures minimal impurities, providing consistent performance in sensitive applications like protein studies, enzyme assays, and cell culture media preparation. Its zwitterionic nature offers excellent buffering capacity between pH 6.1-7.5, while the low metal ion content prevents interference in analytical procedures. The powder form allows for flexible concentration adjustments, and its high water solubility (≥100 mg/mL) enables easy preparation of working solutions. ACES is particularly valued for its resistance to enzymatic degradation and minimal interaction with biological molecules, ensuring reliable experimental reproducibility. The extra pure grade undergoes rigorous quality control to meet strict laboratory standards, making it a preferred choice for researchers requiring precision in their biochemical protocols.

FAQs

1. What is the exact pH range where ACES buffer is most effective?

ACES provides optimal buffering between pH 6.1 and 7.5, with its pKa at 6.8, making it particularly suitable for physiological pH applications.

2. Can this ACES buffer be used for cell culture applications?

Yes, the extra pure grade is specifically formulated to be non-toxic to cells, making it compatible with mammalian cell culture systems when prepared at appropriate concentrations.

3. How does the purity level of 99% affect experimental results?

The 99% purity ensures minimal interference from contaminants like heavy metals or organic impurities, which is critical for sensitive assays and accurate analytical measurements.

4. What is the recommended storage condition for ACES powder?

Store the powder in a tightly sealed container at room temperature, protected from moisture and light, to maintain its stability and shelf life.

5. Is this ACES buffer compatible with common laboratory detergents?

ACES is generally compatible with mild non-ionic detergents like Triton X-100, but compatibility should be verified for specific detergent concentrations in your protocol.