Specifications Table
Product Name – ACES, 99+%, for biochemistry Quantity/Pack Size – 1KG, 100GR, 250GR Form – Powder Grade – Biochemistry Grade Application – pH buffering, protein studies, molecular biology
Product Overview
ACES (N-(2-Acetamido)-2-aminoethanesulfonic acid) is a high-purity zwitterionic buffer with a pKa of 6.8, making it ideal for maintaining stable pH in biochemistry and molecular biology applications. This 99+% grade ACES buffer is synthesized under stringent quality controls to minimize impurities, ensuring reliable performance in sensitive assays, protein purification, and enzyme reactions. Its excellent solubility in water and low metal ion binding properties reduce interference in experimental protocols, while its resistance to enzymatic degradation enhances reproducibility. The powdered form allows for flexible preparation of solutions at varying concentrations, catering to diverse research needs. Compatible with most biological systems, ACES is particularly useful in cell culture media, electrophoresis buffers, and diagnostic kits where precise pH control is critical. The product undergoes rigorous testing for heavy metals, endotoxins, and microbial contamination, meeting international laboratory standards. Store in a cool, dry place to maintain stability and extend shelf life.
FAQs
1. What is the solubility of ACES buffer in water?
ACES buffer is highly soluble in water, typically dissolving at concentrations up to 500 mM (≈10% w/v) under standard laboratory conditions.
2. Can ACES buffer be used for cell culture applications?
Yes, ACES is biocompatible and commonly used in cell culture media to maintain physiological pH without toxicity to mammalian cells.
3. How does ACES compare to HEPES buffer?
ACES has a lower pKa (6.8 vs. HEPES’ 7.5), making it better suited for applications requiring buffering near neutral pH, while HEPES is preferred for slightly alkaline conditions.
4. What is the recommended storage temperature for ACES powder?
Store ACES powder at room temperature (15–25°C) in a tightly sealed container to prevent moisture absorption and degradation.
5. Is this ACES buffer suitable for electrophoresis?
Yes, its low ionic strength and minimal UV absorbance make it ideal for use in electrophoresis buffers, particularly for protein and nucleic acid separation.