Description

Specifications Table

Product Name – ACRIDINE ORANGE HEMI (ZINC CHLORIDE) SALT For Microscopical Staining
Quantity/Pack Size – 10 g, 25 g
Form – Powder
Grade – Laboratory Grade
Application – Fluorescent staining of nucleic acids, microscopy

Product Overview

Acridine Orange hemi (zinc chloride) salt is a versatile fluorescent dye widely used in microscopy for staining nucleic acids. This cationic dye binds selectively to DNA and RNA, emitting distinct fluorescence under UV light, making it invaluable for visualizing cellular structures. The zinc chloride-stabilized form ensures enhanced stability and consistent performance in staining protocols. Its high purity and solubility in water and organic solvents allow for precise preparation of staining solutions. The dye’s metachromatic properties enable differentiation between double-stranded DNA (green fluorescence) and single-stranded RNA (red fluorescence), providing clear contrast in cellular imaging. Ideal for applications requiring high sensitivity and specificity, this product adheres to strict laboratory standards, ensuring reproducible results. Proper storage in a cool, dry place preserves its efficacy, while its compatibility with standard microscopy techniques makes it a staple in research and diagnostic labs.

FAQs

1.

What is the primary use of Acridine Orange hemi (zinc chloride) salt?

Acridine Orange is primarily used as a fluorescent dye for staining nucleic acids in microscopy, helping visualize DNA and RNA in cells. 2.

Is this product compatible with standard fluorescence microscopes?

Yes, it is fully compatible with most fluorescence microscopes, emitting distinct green and red fluorescence for DNA and RNA, respectively. 3.

What is the shelf life of this dye when stored properly?

When stored in a cool, dry place away from light, the dye remains stable for up to 2 years without significant degradation. 4.

Can this stain be used for live-cell imaging?

While primarily used for fixed cells, it can be adapted for live-cell staining under optimized conditions to minimize cytotoxicity. 5.

Are there any safety precautions to consider when handling this dye?

Yes, it should be handled with gloves and protective eyewear, as it is a potential mutagen and irritant. Avoid inhalation and direct skin contact.

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