Description
Specifications Table
Product Name – ACRYLAMIDE 98.5% For Electrophoresis
Quantity/Pack Size – 25 g, 100 g, 500 g
Form – Crystalline Powder
Grade – Electrophoresis Grade
Application – Polyacrylamide gel electrophoresis (PAGE), protein/DNA separation
Product Overview
Acrylamide 98.5% for electrophoresis is a high-purity, lab-grade chemical designed for precise polyacrylamide gel formation in molecular biology applications. This ultra-pure formulation ensures minimal impurities, reducing background noise and enhancing gel clarity for accurate protein and nucleic acid separation. The crystalline powder dissolves uniformly, providing consistent polymerization for reproducible results in SDS-PAGE, native PAGE, and DNA sequencing applications. Its high molecular weight and low conductivity make it ideal for creating gels with optimal pore sizes, facilitating sharp band resolution. The product undergoes stringent quality control to meet electrophoresis-grade standards, ensuring reliability in critical research workflows. Its stability under standard lab conditions and compatibility with common buffer systems make it a versatile choice for routine and high-sensitivity applications. The 98.5% purity level minimizes the risk of artifact formation, while the fine particle size enables rapid dissolution without clumping. This acrylamide is particularly suited for applications requiring high-resolution separation, such as protein fingerprinting or DNA fragment analysis.
FAQs
1. What is the shelf life of acrylamide 98.5% for electrophoresis?
When stored properly in a cool, dry place away from light, unopened acrylamide maintains its stability for up to 2 years from the date of manufacture. Always check the batch-specific expiration date on the label.
2. Can this acrylamide be used for both protein and DNA electrophoresis?
Yes, this electrophoresis-grade acrylamide is suitable for both protein separation via SDS-PAGE and DNA fragmentation analysis. The high purity ensures minimal interference with either application.
3. What precautions should be taken while handling acrylamide powder?
Acrylamide is a neurotoxin in its monomeric form. Always wear nitrile gloves, a lab coat, and safety goggles when handling. Work in a fume hood and avoid inhaling dust. The polymerized form is considered non-toxic.
4. Is this acrylamide compatible with bis-acrylamide for gel preparation?
This product is fully compatible with N,N’-methylenebisacrylamide (bis-acrylamide) for cross-linking in standard polyacrylamide gel recipes. The typical ratio used is 29:1 acrylamide to bis-acrylamide for most applications.
5. How should acrylamide waste be disposed of?
Unpolymerized acrylamide should be treated as hazardous waste. Polymerize any leftover solution with excess APS and TEMED before disposal according to your institution’s chemical waste protocols. Never dispose of acrylamide in regular trash or drains.
