Description
Specifications Table
Product Name – AGAROSE (M) For Electrophoresis
Quantity/Pack Size – 10 g, 25 g, 100 g
Form – Powder
Grade – Molecular Biology
Application – DNA/RNA electrophoresis, nucleic acid separation
Product Overview
AGAROSE (M) For Electrophoresis is a high-performance, low-electroendosmosis (EEO <0.13) agarose designed for precise nucleic acid separation in molecular biology applications. Its refined formulation delivers exceptional gel clarity and mechanical strength (1200 g/cm²), ensuring consistent band resolution across a wide range of DNA/RNA fragment sizes. The product undergoes rigorous purification to eliminate inhibitors, making it compatible with downstream enzymatic reactions. Its optimized gelling temperature (36°C) and melting point (88°C) provide ease of handling while maintaining structural integrity during electrophoresis. The medium EEO profile balances migration speed and resolution, particularly effective for fragments between 50 bp and 20 kb. Each batch undergoes strict quality control to guarantee lot-to-lot consistency, with DNase/RNase-free certification for sensitive applications. The powder form dissolves completely without clumping, producing homogeneous gels with minimal background fluorescence. Its neutral pH (6.5-7.5 in 1% solution) prevents sample degradation during prolonged runs.
FAQs
1. What is the typical gel concentration range for this agarose?
This agarose performs optimally between 0.7% and 2% concentrations for most DNA/RNA applications.
2. How does the EEO value affect electrophoresis results?
Lower EEO values reduce background smearing and improve band sharpness, particularly for smaller nucleic acid fragments.
3. Can this agarose be used for pulsed-field gel electrophoresis?
While suitable for standard electrophoresis, specialized high-molecular-weight agarose is recommended for PFGE applications.
4. What is the recommended storage temperature?
Store at room temperature (15-25°C) in a dry environment to maintain product integrity.
5. Does this agarose contain any additives?
The product is free from additives, preservatives, or buffering agents to ensure compatibility with sensitive applications.
