Description
Specifications Table
| Product Name | Agarose (M) Electrophoresis | 00790 | Loba Chemie |
|---|---|
| Quantity/Pack Size | 100g |
| Form | Powder |
| Grade | Electrophoresis (Low EEO) |
| Application | DNA/RNA gel electrophoresis, molecular biology |
Product Description
Agarose (M) Electrophoresis from Loba Chemie is a premium-grade polysaccharide designed for high-resolution nucleic acid separation. With its low electroendosmosis (EEO) properties, this agarose minimizes background interference, delivering crisp, well-defined bands for DNA and RNA analysis. Whether you’re a student setting up your first gel or a researcher optimizing protocols, this agarose ensures consistent performance in horizontal gel electrophoresis.
Unlike standard agarose, the molecular biology grade of this product undergoes rigorous purification to remove impurities like nucleotides, proteins, and ions that could affect migration patterns. This makes it ideal for applications requiring high sensitivity, such as PCR product analysis, plasmid DNA separation, and RNA integrity checks. The powder form allows for easy preparation of gels at varying concentrations (0.5%–2%), tailored to your specific fragment size needs.
For labs prioritizing reproducibility, Agarose (M) offers batch-to-batch consistency, reducing variability in experimental results. Its compatibility with common buffers (TAE, TBE) and stains (ethidium bromide, SYBR Safe) further simplifies workflow integration. Whether you’re troubleshooting smudged bands or scaling up for high-throughput experiments, this agarose provides the reliability expected in academic and research settings.
Upgrade your electrophoresis setup with high-purity agarose—order online today for precise, publishable results.
Key Applications
This agarose is optimized for:
- DNA fragment analysis (50 bp–20 kb)
- RNA integrity verification
- PCR product confirmation
- Plasmid DNA isolation checks
- Educational lab demonstrations
Preparation Guidelines
To prepare a 1% gel, dissolve 1g of agarose in 100ml of 1X TAE or TBE buffer. Heat the mixture in a microwave until fully dissolved (avoid boiling), then cool to ~60°C before pouring. For best results, use fresh buffers and avoid overheating, which can degrade the agarose. Gels can be stored at 4°C for short-term use but are best cast fresh for critical applications.
Safety and Handling
While agarose is non-toxic, handle the powder in a well-ventilated area to avoid inhalation. Use appropriate PPE (gloves, lab coat) when preparing gels, especially when working with hot solutions or staining agents. Dispose of waste gels according to institutional biohazard protocols if contaminated with nucleic acids.
FAQs
What is the difference between standard and low EEO agarose?
Low EEO agarose undergoes additional purification to reduce charged impurities, minimizing background interference during electrophoresis. This results in sharper bands and more accurate sizing, critical for molecular biology applications.
Can this agarose be used for protein electrophoresis?
No, agarose is not suitable for protein separation due to its large pore size. For proteins, consider polyacrylamide gels or specialized agarose blends designed for protein applications.
How should I store unused agarose powder?
Store the powder in a cool, dry place away from moisture. Reseal the original packaging or transfer to an airtight container to prevent clumping. Properly stored agarose remains stable for years.
Why are my DNA bands smudged when using this agarose?
Smudged bands often result from overheating during gel preparation, old buffers, or excessive DNA loading. Ensure the agarose cools to ~60°C before pouring, use fresh buffers, and optimize sample volumes.
Is this agarose compatible with fluorescent DNA stains?
Yes, it works well with ethidium bromide, SYBR Safe, and other common nucleic acid stains. Follow the stain manufacturer’s guidelines for optimal sensitivity and safety.
