Description
Specifications Table
| Product Name | Alamar Blue? (Resazurin Sodium Salt) | TC235-1G | Himedia |
|---|---|
| Quantity/Pack Size | 1 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Cell viability assays, microbial growth detection, cytotoxicity testing |
Product Description
What is Alamar Blue (Resazurin Sodium Salt)?
Alamar Blue, also known as Resazurin Sodium Salt, is a widely used redox indicator for assessing cell viability and proliferation. This non-toxic, water-soluble dye changes color in response to metabolic activity, making it an essential tool in microbiology, cell biology, and drug discovery research.
How Does Alamar Blue Work?
The dye starts as a blue, non-fluorescent compound (resazurin) and is reduced by viable cells into a pink, fluorescent compound (resorufin). The color change can be measured spectrophotometrically or fluorometrically, providing quantitative data on cell health.
Key Applications of Alamar Blue
This versatile reagent is used in various laboratory applications, including:
- Cell viability and cytotoxicity assays
- Microbial growth detection
- Drug screening and toxicity studies
- Antimicrobial susceptibility testing
How to Use Alamar Blue in Your Experiments
Using Alamar Blue is straightforward. Simply prepare a working solution, add it to your cell culture, and incubate. The color change indicates metabolic activity, allowing you to quantify cell viability. For best results, follow the manufacturer’s protocol or consult standard laboratory guidelines.
Safety and Handling
While Alamar Blue is non-toxic, it’s important to handle all laboratory chemicals with care. Wear appropriate personal protective equipment (PPE) such as gloves and lab coats. Store the reagent in a cool, dry place away from direct sunlight.
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FAQs
1. What is the difference between Alamar Blue and Resazurin Sodium Salt?
Alamar Blue is a commercial formulation of Resazurin Sodium Salt, often optimized for specific applications. Both function as redox indicators but may differ in purity and additional components.
2. Can Alamar Blue be used for bacterial viability testing?
Yes, Alamar Blue is commonly used to assess bacterial viability and growth, making it useful in microbiology research.
3. How should I store Alamar Blue reagent?
Store the powder at room temperature in a tightly sealed container. Once reconstituted, follow the manufacturer’s storage recommendations.
4. Is Alamar Blue toxic to cells?
No, Alamar Blue is non-toxic and does not interfere with cell metabolism, making it ideal for long-term viability studies.
5. Where can I buy authentic Alamar Blue in India?
You can buy genuine Alamar Blue (Resazurin Sodium Salt) online from eqipped, ensuring quality and fast delivery across India.
