Description
Specifications Table
| Product Name | B.G. Sulpha Agar (Brilliant Green Sulpha Agar) |
|---|---|
| Pack Size | 100 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Selective isolation of Salmonella species |
Product Description
B.G. Sulpha Agar, also known as Brilliant Green Sulpha Agar, is a specialized microbiological medium designed for the selective isolation of Salmonella species from clinical and environmental samples. This medium combines the inhibitory effects of brilliant green dye and sulphonamide to suppress the growth of non-target bacteria, allowing Salmonella colonies to thrive and become easily identifiable.
Brilliant green inhibits gram-positive bacteria, while sulphonamide targets other gram-negative organisms, creating an optimal environment for Salmonella detection. The medium is widely used in clinical laboratories, food safety testing, and research settings where accurate bacterial identification is critical.
Himedia’s B.G. Sulpha Agar (M492-100G) is formulated to meet strict quality standards, ensuring consistent performance in microbiological applications. Whether you’re a student, researcher, or educator, this medium provides reliable results for your experiments.
Need a reliable medium for Salmonella isolation? Buy Brilliant Green Sulpha Agar online from eqipped today.
How to Use B.G. Sulpha Agar
To prepare B.G. Sulpha Agar, suspend 100 grams of the powder in 1 liter of distilled water. Heat gently while stirring until fully dissolved. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and pour into sterile Petri dishes. Allow the agar to solidify before inoculation.
For best results, incubate inoculated plates at 35-37°C for 18-24 hours. Salmonella colonies typically appear as colorless or pale pink colonies, while other bacteria are inhibited.
Applications of Brilliant Green Sulpha Agar
This medium is essential for various microbiological applications, including:
- Clinical diagnostics for Salmonella detection
- Food and water safety testing
- Environmental monitoring
- Educational microbiology experiments
Why This Medium Matters
Salmonella infections remain a significant public health concern worldwide. Accurate detection is crucial for preventing outbreaks and ensuring food safety. B.G. Sulpha Agar provides a cost-effective and reliable method for isolating Salmonella, making it a staple in microbiology labs.
Looking for high-quality sulpha agar media? Shop now at eqipped for fast delivery.
FAQs
What is the shelf life of B.G. Sulpha Agar?
The powdered medium has a shelf life of 24 months when stored in a cool, dry place away from direct sunlight.
Can this medium be used for other bacteria besides Salmonella?
While primarily designed for Salmonella, some related gram-negative bacteria may grow, but the medium’s selective properties significantly reduce their presence.
What safety precautions should I take when handling this medium?
Wear appropriate lab attire, including gloves and goggles. Avoid inhalation of the powder and follow standard microbiological safety protocols.
How should I store prepared agar plates?
Store prepared plates at 2-8°C in sealed containers to prevent contamination. Use within 7 days of preparation for optimal results.
Is this medium suitable for student laboratory exercises?
Yes, B.G. Sulpha Agar is commonly used in educational settings for teaching bacterial isolation techniques and Salmonella detection methods.
