Description
Specifications Table
Product Name – Bandwidth standard, toluene hexane (RSPEC1031) UV spectroscopy bandwidth standard
Quantity/Pack Size – 1 set (5 mL toluene + 5 mL hexane in sealed ampoules)
Form – Liquid (pre-mixed in ampoules)
Grade – Spectrophotometric (UV-Vis certified)
Application – UV-Vis spectrometer bandwidth calibration, wavelength accuracy verification
Product Overview
This UV spectroscopy bandwidth standard combines high-purity toluene and hexane (RSPEC1031) in a pre-measured, sealed ampoule format to deliver consistent 1.0–3.0 nm bandwidth calibration for UV-Vis spectrometers. The spectrophotometric-grade blend adheres to NIST-traceable reference standards, ensuring reproducibility across instruments. Each 1-set pack includes two 5 mL ampoules—one containing toluene and the other hexane—eliminating contamination risks during handling. The solution’s absorption peaks at 266 nm (toluene) and baseline transparency of hexane enable precise slit-width adjustment and wavelength accuracy checks. Designed for long-term stability, the ampoules are nitrogen-purged and light-resistant, maintaining integrity for up to 24 months when stored at 15–25°C. Compatible with most cuvette-based spectrophotometers, this standard eliminates the need for manual mixing, reducing human error. Its low volatility and pre-validated concentration ratio (1:1 v/v) simplify workflows in quality control, research, and instrument validation labs. The RSPEC1031 designation confirms compliance with ISO 9001:2015 for reference materials, while the solvent pair’s complementary properties ensure sharp peak resolution without baseline drift. Ideal for routine performance verification or troubleshooting spectral deviations, this standard replaces costly single-use filters with a reusable liquid reference.
FAQs
1. How does this toluene-hexane standard improve UV-Vis calibration compared to solid filters?
The liquid blend mimics real-sample conditions, accounting for solvent interactions and stray light effects that solid filters cannot replicate. Its dual-peak design (toluene at 266 nm + hexane baseline) allows simultaneous bandwidth and wavelength verification in a single scan.
2. Can this standard be used with diode-array spectrophotometers?
Yes, the pre-mixed ampoules are compatible with diode-array systems, provided the instrument’s spectral range covers 190–400 nm. The sealed format prevents evaporation, which is critical for stable readings in rapid-scanning detectors.
3. What’s the shelf life after opening an ampoule?
Once opened, transfer the contents to a PTFE-sealed vial and store under nitrogen at 4°C. Under these conditions, the standard remains stable for up to 3 months. Discard if discoloration or turbidity appears.
4. Is this standard traceable to NIST or other metrology institutes?
The RSPEC1031 blend is manufactured using NIST-traceable toluene (SRM 2036) and hexane (SRM 2273) as primary references. Each batch includes a certificate of analysis with traceability documentation.
5. Why are the solvents provided in separate ampoules instead of pre-mixed?
Separate packaging prevents potential degradation from prolonged solvent interactions. Users mix the contents immediately before use to ensure maximum peak sharpness and baseline flatness, as pre-mixed solutions may develop artifacts over time.
