Description
Specifications Table
Product Name – BARFOED’S REAGENT
Quantity/Pack Size – 250 ml
Form – Liquid
Grade – Laboratory Grade
Application – Monosaccharide detection, qualitative sugar analysis
Product Overview
Barfoed’s reagent is a specialized copper-based solution designed for the selective detection of monosaccharides in laboratory settings. This high-purity reagent reacts with reducing sugars under controlled heating, producing a characteristic red precipitate of copper(I) oxide, confirming the presence of monosaccharides such as glucose, fructose, or galactose. The formulation ensures minimal interference from disaccharides or polysaccharides, making it a reliable choice for qualitative sugar analysis. The reagent is prepared using analytical-grade copper acetate and acetic acid, ensuring consistent performance and reproducibility in tests. Its stability under standard lab conditions allows for long-term storage without degradation, provided it is kept in a tightly sealed container away from direct sunlight. The solution is compatible with standard glassware and does not require additional buffers or stabilizers for routine applications. Whether used in educational demonstrations or research protocols, this reagent delivers clear, interpretable results due to its optimized copper concentration and pH balance. The 250 ml pack size offers sufficient volume for repeated testing while minimizing waste, making it cost-effective for frequent use.
FAQs
1. What is the shelf life of Barfoed’s reagent when stored properly?
When stored in a cool, dark place with the cap tightly sealed, Barfoed’s reagent remains stable for up to 24 months from the date of manufacture. Avoid exposure to moisture or extreme temperatures to prevent premature degradation.
2. Can this reagent distinguish between glucose and fructose?
The reagent detects the presence of monosaccharides but does not differentiate between specific types like glucose and fructose. Additional tests, such as enzyme-specific assays or chromatography, are required for identification.
3. Is Barfoed’s reagent compatible with plastic labware?
The acetic acid in the solution may react with certain plastics over time. For best results, use borosilicate glass containers or high-density polyethylene (HDPE) bottles labeled as acid-resistant.
4. What safety precautions should be taken while handling this reagent?
Wear nitrile gloves, safety goggles, and a lab coat to avoid skin or eye contact. The solution contains copper acetate, which is harmful if ingested or inhaled. Work in a well-ventilated area or under a fume hood.
5. How does the concentration of sugar affect the test results?
Higher sugar concentrations produce a more rapid and intense red precipitate, while dilute solutions may require longer heating times. For consistent results, standardize sample volumes and heating durations across tests.
