Description
Specifications Table
Product Name – BES, 99+%, for biochemistry
Quantity/Pack Size – 25GR, 100GR, 500GR
Form – Powder
Grade – 99+%
Application – Biochemistry, molecular biology, cell culture
Product Overview
BES (N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) is a high-purity zwitterionic buffer with a pKa of 7.15 at 25°C, making it ideal for biological systems requiring precise pH control. This 99%+ grade ensures minimal impurities, providing reliable performance in sensitive biochemical applications. Its excellent water solubility and low toxicity make it suitable for cell culture media, enzyme assays, and protein studies. The powder form allows for flexible preparation of solutions at desired concentrations. BES maintains stable buffering capacity across a wide temperature range, ensuring consistent results in experimental protocols. Its chemical stability prevents degradation during storage, while the sulfonic acid group contributes to its resistance to enzymatic hydrolysis. This buffer is particularly valuable in systems where amine-based buffers may interfere with biological processes. The high purity level minimizes background interference in analytical techniques, making it a preferred choice for researchers demanding precision in their experimental setups.
FAQs
1. What is the effective pH range for BES buffer?
BES provides optimal buffering between pH 6.4 and 7.8, with its pKa at 7.15, making it particularly useful for physiological pH applications.
2. How should BES buffer be stored for long-term stability?
Store the powder in a tightly sealed container at room temperature, protected from moisture and direct sunlight to maintain its stability and purity.
3. Is BES compatible with common biological assays?
Yes, BES is compatible with most biological assays due to its low reactivity and minimal interference with biochemical processes.
4. What are the key advantages of using BES over other buffers?
BES offers excellent temperature stability, low toxicity, and minimal interaction with metal ions compared to many alternative buffers.
5. Can BES be used in cell culture applications?
BES is suitable for cell culture as it maintains stable pH in CO2 environments and exhibits low cytotoxicity at typical working concentrations.
