Description
Specifications Table
| Product Name | BES Buffer 99% for Biochemistry | 1927D | Loba Chemie |
|---|---|
| Pack Size | 100g |
| Form | Powder |
| Grade | Biochemistry Grade |
| Application | pH buffering in biological assays, protein studies, and molecular biology |
Product Description
BES Buffer (N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) is a zwitterionic buffer widely used in biochemistry and molecular biology. With a pKa of 7.15 at 25°C, it provides excellent buffering capacity in the physiological pH range (6.4–7.8), making it ideal for cell culture, enzyme assays, and protein purification.
This high-purity 99% grade ensures minimal interference in sensitive experiments. Whether you’re working on DNA/RNA studies, protein crystallization, or biochemical assays, BES Buffer maintains stable pH conditions, enhancing reproducibility in your research.
At eqipped, we source this buffer from Loba Chemie, a trusted name in laboratory chemicals, ensuring consistent quality for your critical applications. Need bulk quantities for your lab? Contact us for customized solutions.
Key Applications
BES Buffer is commonly used in:
- Cell culture media preparation
- Enzyme activity assays
- Protein crystallization and purification
- Electrophoresis and blotting techniques
- Biochemical research requiring stable pH
Why BES Buffer?
Unlike traditional buffers like Tris or phosphate, BES offers several advantages:
It has minimal metal ion chelation, reducing interference in enzymatic reactions. Its zwitterionic nature ensures low membrane permeability, making it suitable for cell-based assays. Additionally, BES is non-toxic and compatible with most biological systems.
For researchers and students working in biochemistry labs, this buffer is a reliable choice for experiments requiring precise pH control. Buy BES Buffer online today and experience seamless delivery across India.
Handling and Storage
Store BES Buffer in a cool, dry place away from direct sunlight. Keep the container tightly sealed to prevent moisture absorption. For long-term storage, desiccants can be used to maintain stability.
When preparing solutions, use deionized water and adjust pH as needed with HCl or NaOH. Always wear appropriate PPE (gloves, lab coat) when handling chemicals.
Risk Note
While BES Buffer is generally low-hazard, avoid inhalation or direct contact with skin/eyes. In case of exposure, rinse thoroughly with water and seek medical advice if irritation persists.
FAQs
What is the pH range of BES Buffer?
BES Buffer has an effective buffering range of 6.4–7.8, with a pKa of 7.15 at 25°C.
Can BES Buffer be used in cell culture?
Yes, BES is non-toxic and commonly used in cell culture media to maintain stable pH conditions.
How do I prepare a 1M BES Buffer solution?
Dissolve 213.25g of BES powder in 1L of deionized water, then adjust pH with HCl or NaOH as required.
Is BES Buffer compatible with DNA/RNA experiments?
Absolutely. Its low metal ion binding makes it ideal for nucleic acid research without interference.
Where can I buy BES Buffer online in India?
You can purchase high-quality BES Buffer from eqipped with fast shipping and competitive pricing.
