Description
Specifications Table
Product Name – BES sodium salt, 99%
Quantity/Pack Size – 25GR, 100GR, 500GR
Form – Powder
Grade – 99%
Application – Buffer preparation, cell culture, protein studies
Product Overview
BES sodium salt (N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt) is a high-purity zwitterionic buffer with a pKa of 7.15, making it ideal for maintaining stable pH in biological systems. This 99% grade reagent offers exceptional chemical stability, low metal ion binding, and minimal interference in enzymatic reactions. Its water-soluble nature ensures easy dissolution without residue, while the sodium salt form enhances compatibility with physiological conditions. The buffer’s resistance to temperature fluctuations and lack of absorbance in UV-Vis spectroscopy make it particularly valuable for spectrophotometric applications. Unlike traditional buffers, BES maintains its buffering capacity across a broad temperature range (20-30°C) without significant pKa shifts. The powder form allows for precise weighing and custom concentration preparation, while its non-toxic profile ensures safe handling in laboratory environments. This reagent is synthesized through controlled sulfonic acid derivatization followed by sodium neutralization, resulting in a product with consistent lot-to-lot performance and minimal batch variation.
FAQs
1. What is the effective pH range for BES sodium salt?
The useful buffering range for BES sodium salt is between pH 6.4 and 7.8, with optimal performance at pH 7.15 (25°C).
2. Can this buffer be used with divalent cations?
BES sodium salt exhibits low affinity for divalent cations like Ca²⁺ and Mg²⁺, making it suitable for systems requiring these ions.
3. What are the storage recommendations?
Store the powder in a tightly sealed container at room temperature, protected from moisture and direct light. Prepared solutions should be refrigerated at 4°C.
4. How does BES compare to HEPES buffer?
BES has a slightly lower pKa (7.15 vs 7.55 for HEPES) and shows less temperature sensitivity in the physiological pH range.
5. Is this product suitable for cell culture applications?
The high purity and low cytotoxicity of this 99% grade BES sodium salt make it appropriate for most mammalian cell culture systems.
