Description
Specifications Table
| Product Name | BICINE 99% Extra Pure | 01928 | Loba Chemie |
|---|---|
| Quantity/Pack Size | 100g |
| Form | Powder |
| Grade | Extra Pure |
| Application | Biochemistry, Molecular Biology, Protein Research |
Product Description
BICINE (N,N-Bis(2-hydroxyethyl)glycine) is a highly stable buffer widely used in biochemistry and molecular biology. With a pKa of 8.3, it is ideal for maintaining pH in protein purification, enzyme assays, and cell culture media. The 99% extra pure grade ensures minimal impurities, making it suitable for sensitive applications.
Researchers and students rely on BICINE for its non-toxicity and compatibility with biological systems. Whether you’re working in a university lab or a research facility, this buffer provides consistent performance. Need a reliable bicine buffer supplier? eqipped offers lab-grade chemicals with fast shipping across India.
Key Applications
BICINE is commonly used in:
- Protein electrophoresis and blotting
- Enzyme kinetics studies
- Cell lysis and extraction buffers
- DNA/RNA stabilization solutions
Why BICINE is Essential for Your Lab
Unlike other buffers, BICINE maintains stability over a broad temperature range, reducing pH drift during experiments. Its zwitterionic nature minimizes interactions with metal ions, ensuring accurate results. For biochemistry labs, this buffer is a cost-effective alternative to Tris or HEPES in certain applications.
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Safety and Handling
While BICINE is generally non-hazardous, standard lab safety practices should be followed. Wear gloves and goggles when handling. Store in a cool, dry place away from direct sunlight. For disposal, follow local regulations for chemical waste.
FAQs
What is the pH range of BICINE buffer?
BICINE has an effective buffering range of 7.6 to 9.0, with a pKa of 8.3 at 20°C.
Can BICINE be used in cell culture media?
Yes, BICINE is biocompatible and often used in cell culture applications where a stable pH is critical.
Is BICINE compatible with metal ions?
BICINE has low metal-chelating properties, making it suitable for experiments involving metal cofactors.
How do I prepare a 1M BICINE solution?
Dissolve 163.17g of BICINE in 1L of deionized water, adjust pH with HCl or NaOH, and sterilize if needed.
Where can I buy authentic BICINE buffer in India?
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