Description
Specifications Table
| Product Name | Bile Esculin Agar (ISO 10273:2003) | M972I-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Microbiology Grade |
| Application | Detection and isolation of enterococci in clinical and environmental samples |
Product Description
Bile Esculin Agar is a specialized microbiological medium designed for the selective isolation and identification of enterococci, particularly in clinical and environmental samples. Formulated according to ISO 10273:2003 standards, this medium contains bile salts and esculin, which inhibit gram-negative bacteria while allowing enterococci to grow and hydrolyze esculin, producing a characteristic black precipitate.
This medium is widely used in microbiology labs, research institutions, and educational settings for routine testing and identification of enterococcal species. Its differential properties make it an essential tool for diagnostic microbiology, ensuring accurate and reliable results.
When preparing Bile Esculin Agar, follow standard microbiological procedures to achieve optimal performance. The medium should be sterilized by autoclaving and cooled to 45-50°C before pouring into Petri dishes. Inoculated plates should be incubated at 35-37°C for 24-48 hours for best results.
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The medium is particularly useful in identifying enterococci in water, food, and clinical specimens, making it a versatile choice for various applications. Its compliance with ISO standards ensures consistency and reliability in testing protocols.
For researchers and students working on microbiology projects, Bile Esculin Agar provides a dependable solution for enterococcal detection. Its ease of use and clear differential characteristics make it a preferred choice in laboratories across India.
FAQs
What is Bile Esculin Agar used for?
Bile Esculin Agar is used for the selective isolation and identification of enterococci in clinical and environmental samples. It helps differentiate enterococci from other bacteria based on esculin hydrolysis.
How do I prepare Bile Esculin Agar?
Dissolve the powder in distilled water, sterilize by autoclaving, cool to 45-50°C, and pour into Petri dishes. Allow the medium to solidify before use.
What is the incubation time for Bile Esculin Agar?
Incubate inoculated plates at 35-37°C for 24-48 hours. Enterococci will produce a black precipitate due to esculin hydrolysis.
Is Bile Esculin Agar suitable for educational labs?
Yes, it is commonly used in educational settings for microbiology training and research projects involving enterococcal identification.
Where can I buy Bile Esculin Agar online in India?
You can buy Bile Esculin Agar online from eqipped, a trusted supplier of high-quality lab supplies in India. Order now for fast delivery.
