Description
Specifications Table
| Product Name | Biolit™ 10x Roseate PCR Buffer with Mg²⁺ |
|---|---|
| Pack Size | 4 × 1.25 ml |
| Form | Liquid |
| Grade | Molecular Biology Grade |
| Application | PCR, Cloning, Gene Expression, Diagnostics |
Product Description
When precision matters in your PCR experiments, the right buffer can make all the difference. The Biolit™ 10x Roseate PCR Buffer with Mg²⁺ is a meticulously formulated solution designed to enhance the performance of your polymerase chain reactions. Whether you’re a student setting up your first PCR or a seasoned researcher optimizing complex assays, this buffer ensures stability, consistency, and high yield in every run.
PCR buffers play a critical role in maintaining the optimal conditions for DNA amplification. The 10X concentration of this buffer allows for easy dilution, giving you flexibility in reaction setup while minimizing pipetting errors. The inclusion of Mg²⁺ ions is particularly important, as magnesium is a cofactor for DNA polymerase, directly influencing enzyme activity and fidelity. Too little Mg²⁺ can lead to poor amplification, while excess can cause nonspecific binding. This buffer strikes the perfect balance, so you can focus on your results rather than troubleshooting.
What sets the Roseate PCR Buffer apart is its compatibility with a wide range of DNA polymerases, including Biolit™ enzymes. This makes it a versatile choice for various applications, from routine PCR to high-throughput screening. The buffer’s formulation also includes stabilizers that prevent degradation during storage, ensuring long-term reliability in your lab.
For students and educators, this buffer simplifies the learning process. Its consistent performance means fewer failed experiments and more time spent analyzing meaningful data. Researchers will appreciate its robustness in demanding applications like cloning, genotyping, and diagnostic testing. Whether you’re working with challenging templates or optimizing reaction conditions, this buffer delivers dependable results every time.
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FAQs
What is the ideal working concentration for this PCR buffer?
The Biolit™ 10x Roseate PCR Buffer with Mg²⁺ is designed to be used at a 1X final concentration. For a standard 50 µL reaction, add 5 µL of the 10X buffer and adjust the remaining components accordingly. Always ensure the Mg²⁺ concentration is optimal for your specific polymerase and template.
Can this buffer be used with non-Biolit polymerases?
Yes, this buffer is compatible with most standard Taq DNA polymerases and high-fidelity enzymes. However, for best results, we recommend checking the manufacturer’s guidelines for your specific polymerase, as some may require additional supplements or adjusted Mg²⁺ levels.
How should I store this buffer to maintain its stability?
Store the buffer at –20°C for long-term use. Avoid repeated freeze-thaw cycles, as this can degrade performance. For frequent use, aliquot the buffer into smaller volumes to minimize exposure to room temperature.
Is this buffer suitable for multiplex PCR?
Absolutely. The Biolit™ 10x Roseate PCR Buffer with Mg²⁺ is
