Description
Specifications Table
| Product Name | Biolit™ dNTP Mix Ultrapure (2.5 mM Each) |
|---|---|
| Quantity/Pack Size | 1 ml |
| Form | Liquid |
| Grade | Ultrapure (Molecular Biology Grade) |
| Application | PCR, qPCR, Sequencing, Cloning, RT-PCR |
Product Description
When precision matters in molecular biology, the quality of your dNTP mix can make or break your experiment. The Biolit™ dNTP Mix Ultrapure (2.5 mM each) is engineered for researchers, students, and educators who demand consistency and reliability in their PCR, sequencing, and cloning workflows. This ready-to-use mix eliminates the hassle of manual preparation, ensuring uniform concentration and minimal contamination risk.
Whether you’re amplifying DNA for a critical research project or teaching students the fundamentals of PCR, this dNTP mix delivers high fidelity and efficiency. The ultrapure grade means fewer inhibitors, higher yield, and cleaner results—every time. No more worrying about batch variability or degradation. Just add your template, primers, and polymerase, and let the science speak for itself.
For labs in India looking to buy dNTP mix online, eqipped offers a seamless experience with authenticated products, competitive pricing, and fast shipping. No compromises, just results.
Why This dNTP Mix Stands Out
Not all dNTP mixes are created equal. Here’s what sets this one apart:
Consistent Performance: Each batch is rigorously tested for purity and concentration, so your reactions stay reproducible. No unexpected failures mid-experiment.
Time-Saving Convenience: Pre-mixed at 2.5 mM each, it’s ready for direct use in most standard protocols. Skip the pipetting errors and focus on your work.
Versatile Applications: Works seamlessly across PCR, qPCR, RT-PCR, and sequencing. A single solution for multiple techniques.
Long Shelf Life: Stable storage at -20°C means you can stock up without worrying about frequent replacements.
From academic labs to biotech startups, this dNTP mix is trusted by professionals who can’t afford inconsistencies. If you’re searching for a reliable dNTP mix for PCR or need an ultrapure dNTP mix at the best price, this is your go-to choice.
Who Should Use This Product?
This dNTP mix is ideal for:
Researchers: Ensuring high-quality amplification for gene expression studies, genotyping, or cloning.
Students & Educators: Perfect for teaching PCR fundamentals with dependable results every time.
Diagnostic Labs: Critical for sensitive assays where accuracy is non-negotiable.
Biotech Startups: Cost-effective without compromising on performance for R&D projects.
No matter your expertise level, this mix simplifies your workflow while maintaining the integrity of your data.
How to Store and Handle
To maximize shelf life and performance:
Storage: Keep at -20°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles by aliquoting if needed.
Handling: Use nuclease-free tips and tubes to prevent contamination. Thaw on ice before use.
Stability: Stable for up to 2 years when stored correctly. Always check for precipitation before use.
Proper handling ensures you get the most out of every microliter, reducing waste and saving costs in the long run.
Common Applications
This dNTP mix is optimized for:
Standard & High-Fidelity PCR: Reliable amplification of DNA fragments up to several kilobases.
Quantitative PCR (qPCR): Low background noise for accurate quantification.
Reverse Transcription PCR (RT-PCR): Efficient cDNA synthesis for gene expression analysis.
DNA Sequencing: Clean, high-quality templates for Sanger or NGS workflows.
Cloning & Mutagenesis: Precise incorporation for error-free constructs.
Whether you’re troubleshooting a stubborn amplification or scaling up a high-throughput project, this mix adapts to your needs.
Troubleshooting Tips
Even with the best reagents, sometimes things go wrong. Here’s how to debug common issues:
No Amplification: Check primer design, template quality, and polymerase activity. Ensure the dNTP mix wasn’t degraded due to improper storage.
Non-Specific Bands: Optimize annealing temperature or reduce dNTP concentration if needed (though this mix is pre-optimized for most protocols).
Low Yield: Verify template concentration and cycle conditions. This mix is formulated for high efficiency, so yields should be robust under standard conditions.
When in doubt, revisit your protocol basics—sometimes the simplest fixes make the biggest difference.
Why eqipped?
At eqipped, we understand that lab supplies aren’t just products—they’re the backbone of your work. That’s why we curate only the most trusted brands, like SRL Chem, and ensure every item meets strict quality standards. When you buy Biolit dNTP mix online from us, you get:
Authentic Products: Sourced directly from manufacturers to guarantee purity and performance.
Competitive Pricing: The best ultrapure dNTP mix price in India without hidden costs.
Fast & Reliable Delivery: Shipped across India with care to maintain product integrity.
Expert Support: Have questions? Our team is here to help, whether it’s about product selection or troubleshooting.
We’re not just a supplier—we’re your lab partner. Focus on your science; we’ll handle the rest.
FAQs
What is the concentration of each dNTP in this mix?
Each dNTP (dATP, dCTP, dGTP, dTTP) is present at 2.5 mM in the mix, making it ideal for most standard PCR protocols without further dilution.
Can I use this dNTP mix for qPCR?
Yes, this ultrapure mix is suitable for qPCR as it’s free from contaminants that could interfere with fluorescence-based detection or amplification efficiency.
How should I aliquot this dNTP mix to avoid freeze-thaw cycles?
Aliquot into smaller volumes (e.g., 50–100 µL) in nuclease-free tubes and store at -20°C. Thaw only what you need for immediate use.
Is this dNTP mix compatible with high-fidelity polymerases?
Absolutely. The high purity ensures compatibility with both standard Taq and high-fidelity polymerases like Pfu or Phusion.
What’s the shelf life of this product?
When stored at -20°C, the mix remains stable for up to 2 years. Always check for any signs of degradation (e.g., color change or precipitation) before use.
