Description
Specifications Table
| Product Name | BioLit® High Fidelity PFU DNA Polymerase (5U/μl) |
|---|---|
| Pack Size | 250 units, 1000 units |
| Form | Liquid |
| Grade | Molecular Biology Grade |
| Application | PCR, Cloning, High-Fidelity DNA Amplification |
Product Description
When accuracy matters in your PCR experiments, BioLit® High Fidelity PFU DNA Polymerase delivers unmatched precision. Designed for researchers and students who demand error-free DNA amplification, this enzyme ensures high-fidelity replication with minimal mutations. Whether you’re working on cloning, mutagenesis, or complex PCR assays, this polymerase provides the reliability you need.
Unlike standard Taq polymerases, PFU DNA polymerase includes 3′ to 5′ exonuclease proofreading activity, significantly reducing errors during DNA synthesis. This makes it the preferred choice for applications where sequence integrity is critical. The 5U/μl concentration offers excellent value, allowing for multiple reactions without compromising performance.
At eqipped, we understand the importance of consistent results in molecular biology. That’s why we source BioLit® High Fidelity PFU DNA Polymerase from trusted manufacturers like SRL Chem, ensuring you get a product that meets rigorous quality standards. Whether you’re a student setting up your first PCR or a seasoned researcher optimizing a cloning protocol, this polymerase is a dependable tool in your lab arsenal.
Upgrade your PCR setup today—buy BioLit® High Fidelity PFU DNA Polymerase online and experience the difference in accuracy.
Why High-Fidelity Polymerase Matters in PCR
Standard DNA polymerases can introduce errors during amplification, leading to mismatches or frame shifts in your sequences. High-fidelity enzymes like PFU polymerase minimize these errors, making them essential for:
- Cloning and subcloning
- Site-directed mutagenesis
- Long PCR applications
- Next-generation sequencing (NGS) library prep
With BioLit® High Fidelity PFU DNA Polymerase, you can trust that your amplified DNA will closely match the original template, saving time and resources in downstream applications.
Applications in Research and Education
This polymerase is widely used in academic and research labs for:
- Amplifying GC-rich templates
- Generating error-free constructs for protein expression
- Validating CRISPR edits
- Teaching PCR techniques in university labs
Its versatility makes it a staple in both routine and specialized molecular biology workflows.
Storage and Handling
To maintain enzyme activity, store the polymerase at -20°C. Avoid repeated freeze-thaw cycles, as this can degrade performance. For best results, aliquot the enzyme upon arrival and keep it in a frost-free environment.
Risk Note
This product is for research use only. Handle with standard laboratory precautions. Avoid skin contact and inhalation. Dispose of waste according to local regulations.
FAQs
What makes PFU DNA polymerase high-fidelity?
PFU polymerase has proofreading activity (3′ to 5′ exonuclease), which corrects misincorporated nucleotides during DNA synthesis, reducing errors.
Can I use this polymerase for standard PCR?
Yes, but it’s optimized for high-fidelity applications. For routine PCR, Taq polymerase may be more cost-effective.
What’s the shelf life of BioLit® High Fidelity PFU DNA Polymerase?
When stored at -20°C, it remains stable for up to 24 months. Always check the expiration date on the label.
Is this polymerase compatible with additives like DMSO?
Yes, it works well with PCR enhancers like DMSO or betaine for GC-rich templates.
Where can I buy BioLit® High Fidelity PFU DNA Polymerase online in India?
You can order it directly from eqipped, with fast shipping across India and competitive pricing.
