Description
Specifications Table
| Product Name | BioLit® High Fidelity PFU DNA Polymerase (5U/μl) with dNTP |
|---|---|
| Pack Size | 250 units, 1000 units |
| Form | Liquid |
| Grade | Molecular Biology Grade |
| Application | PCR, Cloning, Mutagenesis, High-Fidelity Amplification |
Product Description
When precision matters in your PCR experiments, BioLit® High Fidelity PFU DNA Polymerase delivers unmatched accuracy. This enzyme is engineered for researchers and students who demand high-fidelity amplification, especially in cloning, mutagenesis, and other applications where error-free results are critical.
Unlike standard Taq polymerases, PFU DNA polymerase features 3′ to 5′ exonuclease proofreading activity, significantly reducing misincorporation errors. The inclusion of dNTPs in this formulation ensures you have everything needed for immediate use, saving time and minimizing contamination risks.
Whether you’re working on gene cloning, site-directed mutagenesis, or high-accuracy PCR, this polymerase provides the reliability you need. Its robust performance across a wide range of templates makes it a staple in molecular biology labs.
Upgrade your PCR setup with BioLit® High Fidelity PFU DNA Polymerase—order online today for seamless research.
Why High-Fidelity PFU DNA Polymerase?
Standard Taq polymerases are great for routine PCR, but when your experiment requires high accuracy, PFU DNA polymerase is the better choice. Here’s why:
- Proofreading Activity: Corrects misincorporated nucleotides, reducing errors in amplification.
- High Processivity: Efficiently amplifies long and complex templates.
- Thermostable: Withstands repeated thermal cycling without losing activity.
- Includes dNTPs: Ready-to-use formulation for convenience.
For researchers and students working on cloning, mutagenesis, or high-accuracy PCR, this enzyme ensures your results are both reliable and reproducible.
Applications
BioLit® High Fidelity PFU DNA Polymerase is ideal for:
- High-fidelity PCR amplification
- Gene cloning and subcloning
- Site-directed mutagenesis
- Long-range PCR
- Research applications requiring minimal errors
Storage and Handling
Store at -20°C for long-term stability. Avoid repeated freeze-thaw cycles to maintain enzyme activity. Always use sterile techniques to prevent contamination.
Risk Note
This product is for research use only. Handle with care, following standard laboratory safety protocols. Avoid skin contact and inhalation.
FAQs
What makes PFU DNA polymerase better than Taq for cloning?
PFU DNA polymerase has proofreading activity, which corrects errors during amplification. This results in higher accuracy, making it ideal for cloning where sequence fidelity is critical.
Can I use this polymerase for standard PCR?
Yes, but it’s optimized for high-fidelity applications. For routine PCR, Taq polymerase may be more cost-effective.
Does this product include dNTPs?
Yes, the formulation includes dNTPs, so you don’t need to add them separately.
What’s the shelf life of this enzyme?
When stored at -20°C, it remains stable for up to 12 months. Avoid frequent thawing to preserve activity.
Is this suitable for long-range PCR?
Yes, its high processivity makes it effective for amplifying longer DNA fragments.
