Description
Specifications Table
| Product Name | Biolit One Step RT-qPCR Master Mix (2x) |
|---|---|
| Pack Size | 500 µl |
| Form | Liquid |
| Grade | Molecular Biology |
| Application | One-step RT-qPCR, gene expression, viral RNA detection |
Product Description
When you need accurate and efficient RNA detection, the Biolit One Step RT-qPCR Master Mix (2x) delivers reliable results in a single reaction. Designed for researchers, students, and educators, this ready-to-use mix simplifies your workflow by combining reverse transcription and qPCR amplification in one step. Whether you’re analyzing gene expression, quantifying viral loads, or conducting molecular diagnostics, this master mix ensures high sensitivity and reproducibility.
The 2x concentration means you only need to add your RNA template and primers, saving time and reducing pipetting errors. Optimized for a wide range of targets, it works seamlessly with most real-time PCR instruments, making it a versatile choice for labs of all sizes. At eqipped, we understand the importance of precision in molecular biology, which is why we offer this high-quality master mix to support your research needs.
For best results, store the master mix at -20°C and avoid repeated freeze-thaw cycles. Each 500 µl pack provides enough reagent for multiple experiments, ensuring cost-effectiveness without compromising performance. Whether you’re a student setting up your first RT-qPCR experiment or a seasoned researcher, this master mix is a dependable tool for your lab.
Upgrade your RT-qPCR workflow with Biolit’s trusted master mix. Order now and experience seamless RNA detection in every run.
FAQs
What is the shelf life of Biolit One Step RT-qPCR Master Mix?
The master mix is stable for up to 12 months when stored at -20°C. Avoid repeated freeze-thaw cycles to maintain performance.
Can I use this master mix for multiplex RT-qPCR?
Yes, it is compatible with multiplex assays. However, optimize primer concentrations and ensure minimal cross-reactivity for best results.
Is this master mix suitable for detecting low-copy RNA targets?
Absolutely. Its high sensitivity makes it ideal for low-abundance transcripts, but ensure your RNA extraction method is efficient.
Do I need to add separate reverse transcriptase?
No, the master mix contains all necessary enzymes for reverse transcription and qPCR amplification in a single tube.
How do I troubleshoot if my RT-qPCR results are inconsistent?
Check your RNA quality, primer design, and reaction setup. Ensure proper storage of the master mix and avoid template degradation.
