Description
Specifications Table
| Product Name | BioLit® T7 RNA Polymerase (50U/µl) with 10X IVT Buffer |
|---|---|
| Pack Size | 1000 units, 5000 units |
| Form | Liquid |
| Grade | Molecular Biology Grade |
| Application | In vitro transcription (IVT), RNA synthesis, molecular cloning |
Product Description
When it comes to reliable RNA synthesis, BioLit® T7 RNA Polymerase stands out as a top choice for researchers and students alike. This high-purity enzyme, paired with a 10X IVT Buffer, ensures efficient in vitro transcription (IVT) with minimal background noise. Whether you’re working on gene expression studies, RNA probe synthesis, or molecular cloning, this enzyme delivers consistent results every time.
The T7 RNA polymerase is a DNA-dependent RNA polymerase that specifically recognizes the T7 promoter sequence, making it indispensable for generating large quantities of RNA transcripts. The included 10X IVT Buffer is optimized to provide the ideal conditions for transcription, enhancing both yield and fidelity. This makes it perfect for applications like RNA interference (RNAi), RNA structure studies, and even synthetic biology projects.
One of the key advantages of using BioLit® T7 RNA Polymerase is its robustness. Unlike some other polymerases, it maintains high activity even in suboptimal conditions, reducing the risk of failed experiments. The 50U/µl concentration ensures that you get more reactions per unit, making it a cost-effective solution for labs with high throughput needs.
For students and educators, this enzyme is an excellent tool for teaching molecular biology techniques. Its reliability means fewer repeats and more time for analysis. Researchers will appreciate its consistency, especially when working on sensitive applications like CRISPR guide RNA synthesis or viral RNA studies.
Looking to buy T7 RNA polymerase online? eqipped offers this high-quality enzyme at competitive prices, ensuring you get the best value without compromising on performance. Whether you need it for academic research or industrial applications, this product is a trusted choice.
Need bulk quantities? The 1000 units and 5000 units pack sizes cater to both small-scale and large-scale experiments, so you never run out when you need it most.
FAQs
What is the shelf life of BioLit® T7 RNA Polymerase?
The enzyme is stable for 12 months when stored at -20°C. Avoid repeated freeze-thaw cycles to maintain activity.
Can I use this enzyme for in vivo transcription?
No, T7 RNA Polymerase is designed for in vitro applications only. For in vivo studies, consider using cellular RNA polymerases or specialized vectors.
What is the optimal temperature for T7 RNA Polymerase activity?
The enzyme works best at 37°C, though it retains some activity between 30°C and 42°C. Higher temperatures may reduce yield.
How do I calculate the amount of enzyme needed for my reaction?
A typical reaction uses 1-2 units of enzyme per microgram of DNA template. For a 20µl reaction, 1µl of 50U/µl enzyme is usually sufficient.
Is this product compatible with modified nucleotides?
Yes, BioLit® T7 RNA Polymerase can incorporate modified nucleotides (e.g., biotin-UTP, DIG-UTP) for labeling, though efficiency may vary. Test small-scale reactions first.
