Description
Specifications Table
| Product Name | BIS-TRIS 99% Molecular Biology |
|---|---|
| Quantity/Pack Size | 25 g – 100 g |
| Form | Powder |
| Grade | Molecular Biology |
| Application | Electrophoresis buffers, protein research |
Product Description
When precision matters in your lab experiments, BIS-TRIS 99% Molecular Biology from Loba Chemie delivers consistent performance. This high-purity buffer component is a staple in molecular biology labs, particularly for electrophoresis applications where pH stability is critical. Whether you’re separating proteins or nucleic acids, this powder ensures reliable results every time.
BIS-TRIS, or Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane, is a zwitterionic buffer known for its excellent buffering capacity between pH 5.8 and 7.2. Its low UV absorbance makes it ideal for sensitive assays, while its compatibility with common laboratory techniques ensures seamless integration into your workflow.
At eqipped, we understand the demands of research and education. That’s why we offer BIS-TRIS 99% in convenient pack sizes of 25g and 100g, so you can choose the quantity that fits your lab’s needs without compromise. Each batch undergoes rigorous quality checks to meet molecular biology grade standards, giving you confidence in your experimental outcomes.
Upgrade your lab’s buffer solutions today with BIS-TRIS 99% Molecular Biology. Shop now for fast delivery across India.
FAQs
What is BIS-TRIS used for in molecular biology?
BIS-TRIS is primarily used as a buffering agent in electrophoresis, particularly for protein separation in PAGE gels. Its stable pH range makes it ideal for maintaining optimal conditions during gel runs.
How do I prepare a buffer solution with BIS-TRIS powder?
Dissolve the required amount of BIS-TRIS in deionized water, adjust the pH with HCl or NaOH, and bring to the final volume. A typical 1M stock solution can be prepared by dissolving 209.2 g in 1L of water.
Is this BIS-TRIS suitable for DNA electrophoresis?
While BIS-TRIS is more commonly used for protein electrophoresis, it can be used in DNA applications where its pH range is appropriate. For most DNA work, TAE or TBE buffers are more standard.
What’s the difference between BIS-TRIS and regular Tris buffer?
BIS-TRIS has a lower pKa (6.5) compared to Tris (8.1), making it better suited for applications requiring buffering in the slightly acidic to neutral range. It also has less temperature sensitivity.
Can I buy BIS-TRIS in smaller quantities for trial use?
Yes, eqipped offers BIS-TRIS 99% in 25g packs, perfect for small-scale experiments or trial runs before committing to larger quantities.
