Description
Specifications Table
| Product Name | BIS-TRIS Propane Buffer extrapure, 99% | 49084 | SRL Chem |
|---|---|
| Pack Size | 25 Gms, 100 Gms, 500 Gms |
| Form | Powder |
| Grade | Extrapure |
| Application | Electrophoresis, biochemical assays, buffer preparation |
Product Description
BIS-TRIS Propane Buffer is a high-purity biochemical reagent designed for precise pH control in electrophoresis and other sensitive laboratory applications. With a purity of 99%, this extrapure grade buffer ensures minimal interference in experimental results, making it ideal for researchers, students, and educators who demand consistency.
This buffer is commonly used in protein and nucleic acid electrophoresis, where maintaining a stable pH is critical. Its zwitterionic nature provides excellent buffering capacity across a wide pH range, particularly in the physiological range (pH 6.0–9.5). Whether you’re running SDS-PAGE, native PAGE, or other gel-based assays, BIS-TRIS Propane Buffer delivers reliable performance.
At eqipped, we source this product from SRL Chem, a trusted name in laboratory chemicals, ensuring you receive a product that meets stringent quality standards. Available in convenient pack sizes of 25g, 100g, and 500g, it’s perfect for both small-scale experiments and bulk laboratory use.
Need a dependable buffer for your next experiment? Buy BIS-TRIS Propane Buffer online today for accurate, reproducible results.
Key Applications
BIS-TRIS Propane Buffer is versatile and widely used in:
- Protein electrophoresis (SDS-PAGE, native PAGE)
- Nucleic acid separation techniques
- Enzyme assays requiring stable pH conditions
- Buffer preparation for biochemical research
- Cell culture and molecular biology applications
Why This Buffer Stands Out
Unlike generic buffers, BIS-TRIS Propane offers several advantages:
It has a higher buffering capacity compared to traditional Tris buffers, especially in the neutral to slightly alkaline pH range. Its low UV absorbance makes it suitable for spectroscopic studies, and its chemical stability ensures long shelf life when stored properly.
For researchers working on sensitive assays, this buffer minimizes ionic interactions that could affect protein mobility or enzyme activity. Students and educators will appreciate its ease of use and reliability in teaching labs.
Storage and Handling
To maintain the integrity of BIS-TRIS Propane Buffer, store it in a cool, dry place away from direct sunlight. Keep the container tightly sealed to prevent moisture absorption. For long-term storage, consider using desiccants in the storage area.
When preparing solutions, always use high-quality deionized water and follow standard lab protocols for buffer preparation. Proper handling ensures optimal performance in your experiments.
Safety Considerations
While BIS-TRIS Propane Buffer is generally safe for laboratory use, it’s important to follow standard safety practices:
- Wear appropriate personal protective equipment (PPE) such as gloves and goggles.
- Avoid inhalation or ingestion. In case of contact with skin or eyes, rinse thoroughly with water.
- Dispose of waste according to local regulations for chemical waste.
FAQs
What is BIS-TRIS Propane Buffer used for?
It is primarily used as a buffering agent in electrophoresis, particularly for protein and nucleic acid separation. It’s also used in biochemical assays requiring stable pH conditions.
How do I prepare a solution with BIS-TRIS Propane Buffer?
Dissolve the required amount of powder in deionized water, adjust the pH using HCl or NaOH, and sterilize if necessary. Refer to your protocol for specific concentrations.
What is the difference between BIS-TRIS and regular Tris buffer?
BIS-TRIS Propane has a higher buffering capacity in the neutral to alkaline pH range and is less likely to interfere with biological samples compared to traditional Tris buffers.
Can I use this buffer for cell culture applications?
Yes, it is suitable for cell culture media where precise pH control is essential. Ensure proper sterilization before use.
Where can I buy BIS-TRIS Propane Buffer online in India?
You can purchase high-quality BIS-TRIS Propane Buffer from eqipped, a trusted supplier of laboratory chemicals for students, researchers, and educators.
