Description
Specifications Table
| Product Name | Brewer Thioglycollate Medium, Modified |
|---|---|
| Quantity/Pack Size | 100 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Anaerobic bacterial culture, microbiology research |
Product Description
Brewer Thioglycollate Medium is a specialized microbiology culture medium designed for the cultivation of anaerobic and microaerophilic bacteria. This modified version from Himedia (M195A-100G) provides excellent results in laboratory settings, making it a preferred choice for researchers, students, and educators across India.
The medium contains sodium thioglycollate, which helps maintain a low oxidation-reduction potential, creating an ideal environment for anaerobic growth. Its unique formulation supports the growth of both fastidious and non-fastidious microorganisms, making it versatile for various microbiological applications.
Whether you’re conducting research, teaching microbiology, or performing routine lab tests, this medium ensures reliable and consistent results. The 100g pack size is perfect for small to medium-scale laboratory use, offering great value without compromising quality.
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Key Applications
This modified Brewer medium is particularly useful for:
- Cultivating anaerobic bacteria in research labs
- Educational demonstrations of microbial growth
- Clinical microbiology applications
- Quality control testing in pharmaceuticals
- Environmental microbiology studies
Why This Medium Stands Out
The Brewer Thioglycollate Medium from Himedia offers several advantages that make it a top choice among microbiologists:
- Consistent performance for reliable results
- Easy preparation with clear instructions
- Long shelf life when stored properly
- Cost-effective for educational institutions
- Trusted brand with proven quality
Preparation and Usage
To prepare the medium, simply follow these steps:
- Dissolve 29.75 grams of the powder in 1 liter of distilled water
- Heat gently while stirring until completely dissolved
- Dispense into appropriate containers and sterilize by autoclaving at 121°C for 15 minutes
- Cool to room temperature before use
For best results, always follow the manufacturer’s instructions included with the product. Proper preparation ensures optimal growth conditions for your microbial cultures.
Storage Recommendations
Store the unopened powder at room temperature (15-30°C) in a dry place. Once prepared, the medium should be stored at 2-8°C and used within the recommended timeframe. Always check for signs of contamination before use.
Safety Considerations
While this medium is generally safe to handle, it’s important to follow standard laboratory safety protocols:
- Wear appropriate personal protective equipment
- Avoid inhalation of the powder
- Dispose of used medium according to laboratory waste guidelines
- Keep away from incompatible substances
FAQs
What is Brewer Thioglycollate Medium used for?
This medium is primarily used for cultivating anaerobic and microaerophilic bacteria in laboratory settings. It’s particularly useful for isolating and growing organisms that require low oxygen environments.
How do I prepare Brewer Thioglycollate Medium?
Dissolve 29.75 grams of the powder in 1 liter of distilled water, heat gently while stirring, then autoclave at 121°C for 15 minutes. Cool to room temperature before use.
Can I use this medium for aerobic bacteria?
While it’s designed for anaerobic growth, some aerobic bacteria may grow in the upper portion of the medium where oxygen is present. However, it’s not ideal for aerobic culture.
What’s the shelf life of this product?
The unopened powder has a long shelf life when stored properly. Always check the expiration date on the package and follow storage instructions.
Is this medium suitable for educational purposes?
Yes, this medium is excellent for educational settings. Its reliable performance and ease of use make it ideal for teaching microbiology concepts to students.
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