Description
Specifications Table
Product Name – Brij 35 30% Solution
Quantity/Pack Size – 500 ml
Form – Liquid Solution
Grade – Laboratory/Research Grade
Application – Protein Solubilization, Cell Lysis, Chromatography, Emulsification
Product Overview
Brij 35 30% Solution stands as a cornerstone non-ionic polyoxyethylene surfactant, meticulously formulated for the demanding requirements of contemporary laboratory settings. Delivered as a readily usable 30% aqueous solution, it offers unparalleled convenience and dependable performance in a multitude of biochemical and molecular biology applications. Renowned for its mild action, Brij 35 is exceptionally adept at solubilizing proteins and other intricate macromolecules, critically maintaining their structural integrity and biological function. Its role extends to efficient cell membrane disruption for protein extraction, fostering enzyme stability, and significantly improving separation efficiency across various chromatographic methods, including gel filtration and ion exchange. The inherent non-ionic nature of Brij 35 ensures minimal interaction with critical biological processes, preventing interference with enzymatic activities and spectrophotometric analyses, which is vital for obtaining precise and consistent data. Beyond its solubilization capabilities, this solution exhibits superior emulsifying properties, facilitating the creation of stable suspensions and homogeneous dispersions, essential for a diverse array of experimental protocols. This high-purity Brij 35 solution is an invaluable asset for preserving the delicate nature of biological samples, thereby guaranteeing optimal and reproducible experimental results in both advanced research and educational laboratories. Its versatility and reliability make it a preferred choice for professionals seeking consistent performance.
FAQs
1. What are the primary applications of Brij 35 30% Solution in a lab?
Brij 35 30% Solution is primarily used for the gentle solubilization of proteins, cell lysis, and as a component in various chromatographic techniques like gel filtration and ion exchange chromatography. It also serves as an effective emulsifying agent.
2. Is Brij 35 compatible with sensitive enzymatic assays?
Yes, Brij 35 is a non-ionic detergent, which means it generally causes minimal interference with enzymatic activities compared to ionic detergents. This makes it suitable for sensitive enzymatic assays where protein function needs to be preserved.
3. How should Brij 35 30% Solution be stored to maintain its efficacy?
For optimal stability and shelf-life, Brij 35 30% Solution should be stored at room temperature or refrigerated, away from direct sunlight. Ensure the container is tightly sealed to prevent contamination or evaporation.
4. Can Brij 35 be used for nucleic acid extraction?
While Brij 35 is primarily known for protein-related applications, its gentle cell lysis properties can be useful in initial stages of nucleic acid extraction. However, specific protocols may call for other detergents depending on the downstream application.
5. What is the difference between Brij 35 and other common non-ionic detergents?
Brij 35 is a polyoxyethylene ether with a specific hydrophilic-lipophilic balance (HLB) value, making it effective for solubilizing membrane proteins gently. Other non-ionic detergents like Triton X-100 or Tween 20 have different HLB values and may be preferred for distinct applications based on their specific solubilization and micellization properties.
