Description
Specifications Table
| Product Name | Brilliant Green Bile Broth (ISO 4831:2006) | M1211-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Detection of coliforms in water, food, and environmental samples |
Product Description
Brilliant Green Bile Broth is a specialized microbiological medium designed for the selective detection and enumeration of coliform bacteria in water, food, and environmental samples. Formulated in accordance with ISO 4831:2006 standards, this medium inhibits the growth of Gram-positive bacteria while allowing coliforms to thrive, making it an essential tool for quality control in laboratories.
The medium contains bile salts and brilliant green dye, which suppress non-coliform organisms, while lactose serves as the fermentable carbohydrate. Gas production in Durham tubes indicates the presence of coliforms, ensuring accurate and reliable results. Whether you’re testing drinking water, dairy products, or pharmaceutical samples, this broth provides consistent performance.
At eqipped, we ensure that every batch of Brilliant Green Bile Broth meets stringent quality checks, so you can trust the results for your research or compliance testing. The 500 gm pack is ideal for labs with frequent testing needs, offering cost-effective bulk supply without compromising on quality.
Need a reliable medium for coliform detection? Buy Brilliant Green Bile Broth online today for fast, accurate results.
How Brilliant Green Bile Broth Works
This medium leverages a combination of selective and differential agents to isolate coliforms:
- Bile Salts: Inhibit Gram-positive bacteria, allowing only coliforms to grow.
- Brilliant Green Dye: Further suppresses non-coliform organisms.
- Lactose: Fermented by coliforms, producing gas (detected in Durham tubes).
After inoculation and incubation, the presence of gas in the Durham tube confirms coliform contamination, providing a clear and interpretable result.
Applications of Brilliant Green Bile Broth
This medium is widely used in:
- Water quality testing (drinking water, wastewater).
- Food safety analysis (dairy, beverages, processed foods).
- Environmental monitoring (soil, surface water).
- Pharmaceutical and cosmetic microbiological testing.
Its compliance with ISO 4831:2006 ensures it meets international standards for coliform detection, making it a trusted choice for regulatory and research purposes.
Why This Medium is Essential for Your Lab
Accurate coliform detection is critical for public health and safety. Brilliant Green Bile Broth simplifies this process with:
- High selectivity for coliforms, reducing false positives.
- Clear gas production for easy interpretation.
- Consistent performance across diverse sample types.
Whether you’re a student, researcher, or quality control professional, this medium delivers dependable results every time.
Storage and Handling
Store the powder in a cool, dry place away from direct sunlight. Once prepared, the broth should be used immediately or stored at 2-8°C for short-term use. Follow standard microbiological practices for disposal to ensure safety.
FAQs
What is Brilliant Green Bile Broth used for?
It is used to detect and enumerate coliform bacteria in water, food, and environmental samples as per ISO 4831:2006 standards.
How do I prepare Brilliant Green Bile Broth?
Dissolve 50 gm of the powder in 1 liter of distilled water, heat to boiling, and sterilize by autoclaving at 121°C for 15 minutes. Cool before use.
What does a positive result look like?
A positive result is indicated by gas production in the Durham tube, confirming coliform presence.
Can this medium detect E. coli?
While it detects coliforms, further testing (e.g., EMB agar) is needed to confirm E. coli specifically.
Where can I buy Brilliant Green Bile Broth online?
You can purchase it from eqipped, ensuring authentic Himedia products with fast delivery across India.
