Description
Specifications Table
| Product Name | Brilliant Green HiVeg® Broth 2% |
|---|---|
| Quantity/Pack Size | 100 gm |
| Form | Powder |
| Grade | Microbiology Grade |
| Application | Selective enrichment of Salmonella spp. |
Product Description
When it comes to microbiology research, having the right medium is crucial. Brilliant Green HiVeg® Broth 2% from Himedia is a trusted choice for labs across India. This vegetable-based broth is designed to selectively enrich Salmonella species, making it an essential tool for food safety testing, clinical diagnostics, and research applications.
Unlike traditional formulations, this HiVeg® variant replaces animal-derived components with plant-based alternatives, ensuring ethical sourcing without compromising performance. The 2% brilliant green concentration effectively inhibits gram-positive bacteria while allowing Salmonella to thrive, giving you reliable results every time.
Whether you’re a student setting up your first lab experiment or a seasoned researcher conducting critical studies, this broth provides the consistency and selectivity you need. Its powder form allows for easy preparation and long-term storage, making it a practical addition to any microbiology lab.
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Why This Broth Stands Out
The unique formulation of Brilliant Green HiVeg® Broth addresses several key challenges in microbiology labs. First, its selective properties help reduce false positives by suppressing competing flora. Second, the vegetable peptone base ensures batch-to-batch consistency, which is vital for reproducible results. Finally, the inclusion of brilliant green dye provides visual confirmation of bacterial growth patterns.
For educational institutions, this broth serves as an excellent teaching tool. Students can observe firsthand how selective media work in isolating specific pathogens. The clear differentiation between inhibited and growing colonies makes it ideal for practical demonstrations in microbiology courses.
Practical Applications
This broth finds extensive use in:
- Food safety testing for Salmonella contamination
- Clinical microbiology labs for pathogen identification
- Environmental monitoring of water and soil samples
- Academic research on bacterial physiology
- Quality control in pharmaceutical manufacturing
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Preparation and Usage Guidelines
To prepare the medium, dissolve 100 grams of powder in 1 liter of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. For optimal results, maintain the pH between 6.8-7.2. The prepared broth should appear clear green, and any turbidity may indicate contamination or improper preparation.
When inoculating samples, use aseptic techniques to prevent cross-contamination. Incubate at 35-37°C for 18-24 hours. Positive samples will show visible growth, while inhibited cultures will remain clear. Always include appropriate controls for accurate interpretation.
Safety Considerations
While this product is generally safe for lab use, proper handling is recommended. The brilliant green dye may cause skin irritation, so gloves and lab coats should be worn during preparation. Dispose of used cultures according to your institution’s biohazard waste protocols.
FAQs
What is the shelf life of Brilliant Green HiVeg® Broth?
The powder form has a shelf life of 24 months when stored in a cool, dry place away from direct sunlight. Prepared broth should be used within 7 days when stored at 2-8°C.
Can this broth be used for other bacteria besides Salmonella?
While primarily designed for Salmonella, some related Enterobacteriaceae may show growth. However, the brilliant green concentration is optimized specifically for Salmonella selectivity.
How does this compare to traditional brilliant green broth?
The HiVeg® version offers identical performance while being completely animal-component-free, making it suitable for labs with ethical sourcing requirements.
What’s the recommended incubation time?
Standard protocols suggest 18-24 hours at 35-37°C. Extended incubation may lead to overgrowth of non-target organisms.
Where can I buy authentic Himedia Brilliant Green HiVeg® Broth?
eqipped offers genuine Himedia products with verified authenticity. Our platform ensures you receive laboratory-grade supplies with proper documentation.
