Description
Specifications Table
| Product Name | Bromo Cresol Purple Agar w/ Lactose | M1905-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Differential medium for lactose fermentation studies |
Product Description
Bromo Cresol Purple Agar with Lactose is a specialized microbiological medium designed to identify and differentiate bacteria based on their ability to ferment lactose. This agar contains bromo cresol purple as a pH indicator, which changes color in response to acid production during lactose fermentation. It is widely used in research labs, educational institutions, and clinical settings for studying bacterial metabolism.
The medium is prepared by dissolving the powder in distilled water, sterilizing, and pouring into petri dishes. Colonies of lactose-fermenting bacteria produce a yellow color due to acid formation, while non-fermenters remain purple. This clear visual distinction makes it an essential tool for microbiologists.
At eqipped, we ensure this product meets stringent quality standards, providing reliable results for your experiments. Whether you’re a student, researcher, or educator, this agar offers consistent performance for your microbiology needs.
Key Applications
This agar is particularly useful for:
- Identifying lactose-fermenting bacteria in mixed cultures.
- Teaching microbiology concepts in educational labs.
- Research applications requiring differential media.
How to Use
To prepare the medium, follow these steps:
- Dissolve 500 gm of the powder in 1 liter of distilled water.
- Heat gently while stirring to ensure complete dissolution.
- Autoclave at 121°C for 15 minutes to sterilize.
- Cool to 45-50°C and pour into sterile petri dishes.
- Allow the agar to solidify before inoculating with bacterial samples.
Need reliable microbiology media for your lab? Buy Bromo Cresol Purple Agar with Lactose online at eqipped for accurate and consistent results.
Storage and Handling
Store the powder in a cool, dry place away from direct sunlight. Once prepared, the agar plates should be stored at 2-8°C and used within a month for optimal performance. Always follow standard laboratory safety protocols when handling microbiological media.
FAQs
What is the purpose of bromo cresol purple in this agar?
Bromo cresol purple acts as a pH indicator, turning yellow in acidic conditions (lactose fermentation) and remaining purple in neutral or alkaline conditions.
Can this agar be used for clinical diagnostics?
While it is commonly used in research and education, clinical applications should follow specific regulatory guidelines. Consult your lab protocols for diagnostic use.
How long does it take to see results after inoculation?
Visible color changes typically appear within 24-48 hours of incubation at 37°C, depending on the bacterial strain.
Is this product suitable for student labs?
Yes, it is widely used in educational settings due to its clear visual results and ease of interpretation.
Where can I buy bromo cresol purple agar online?
You can purchase high-quality Bromo Cresol Purple Agar with Lactose from eqipped, ensuring authentic Himedia products for your lab.