Description
Specifications Table
Product Name – Bromo Phenol Blue ABT. 1% Solution in DMF
Quantity/Pack Size – 50 ml
Form – Liquid Solution
Grade – Analytical Grade
Application – pH Indicator, Electrophoresis Tracking Dye
Product Overview
The Bromo Phenol Blue ABT. 1% Solution in DMF is a high-quality chemical reagent indispensable for a wide array of laboratory procedures. Prepared at a precise 1% concentration using Dimethylformamide (DMF) as the solvent, this solution ensures stability and optimal performance. Bromo Phenol Blue is well-known for its utility as a pH indicator, exhibiting distinct color changes across a specific pH range, making it invaluable for titrations and determining the acidity or alkalinity of solutions. Its color transition from yellow in acidic conditions to blue-violet in basic conditions provides a clear visual cue for critical experimental endpoints.
Beyond its pH indicator capabilities, this Bromo Phenol Blue solution is also widely employed as a tracking dye in agarose and polyacrylamide gel electrophoresis. Its distinct blue color allows researchers and students to visually monitor the progress of DNA, RNA, or protein separation, ensuring accurate sample migration without interfering with the molecular components. The choice of DMF as a solvent ensures excellent solubility and broad compatibility in various experimental setups where an organic solvent is preferred or required. This analytical grade solution guarantees consistent results, critical for reproducible scientific outcomes. Researchers, students, and educators seeking a dependable and versatile indicator or electrophoresis tracking agent will find this product a cornerstone for their laboratory work. Its purity and carefully controlled concentration make it a reliable choice for achieving precise and accurate experimental data across diverse analytical and biochemical applications, contributing to the success of numerous scientific investigations.
FAQs
1. What is the primary use of Bromo Phenol Blue 1% Solution in DMF?
This solution is primarily used as a pH indicator in titrations and as a tracking dye in gel electrophoresis for visualizing the migration of DNA, RNA, or proteins.
2. Why is DMF (Dimethylformamide) used as the solvent for this solution?
DMF is used to ensure excellent solubility of Bromo Phenol Blue and to provide broad compatibility for applications where an organic solvent is necessary or preferred over an aqueous solution.
3. What are the recommended storage conditions for this Bromo Phenol Blue solution?
It is recommended to store the solution in a cool, dry place, away from direct sunlight and heat, in its original tightly sealed container to maintain its stability and effectiveness.
4. Is this Bromo Phenol Blue ABT. 1% Solution suitable for sensitive analytical work?
Yes, being an ABT. (Analytical Grade) solution, it is manufactured to high purity standards, making it highly suitable for precise analytical procedures and critical laboratory applications.
5. What color change can be observed when using Bromo Phenol Blue as a pH indicator?
Bromo Phenol Blue typically changes color from yellow in acidic conditions (below pH 3.0) to blue-violet in basic conditions (above pH 4.6), providing a clear visual indication of pH changes.
