Description
Specifications Table
| Product Name | Broth Medium I (Tetrathionate Bile-Brilliant Green Broth) (EP) | ME1255-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | EP (European Pharmacopoeia) |
| Application | Selective enrichment of Salmonella spp. in food, water, and clinical samples |
Product Description
Tetrathionate Bile-Brilliant Green Broth is a specialized microbiology culture medium designed for the selective enrichment of Salmonella species. This medium is widely used in food safety testing, water analysis, and clinical microbiology to isolate and identify Salmonella from complex samples.
The formulation includes sodium tetrathionate, which inhibits the growth of many Gram-negative bacteria while allowing Salmonella to thrive. Bile salts and brilliant green further suppress non-target organisms, ensuring high selectivity. This makes it an essential tool for laboratories conducting quality control and pathogen detection.
Whether you’re a student, researcher, or educator, this medium provides reliable results for Salmonella detection. Its compliance with European Pharmacopoeia (EP) standards ensures consistency and accuracy in your experiments. For those looking to buy tetrathionate broth medium online, eqipped offers a trusted source for high-quality lab supplies.
How to Use Tetrathionate Bile-Brilliant Green Broth
1. Reconstitute the powder in distilled water as per the manufacturer’s instructions. 2. Sterilize the medium by autoclaving at 121°C for 15 minutes. 3. Inoculate the sample into the broth and incubate at 35-37°C for 18-24 hours. 4. Subculture onto selective agar for further identification.
For best results, follow standard microbiological practices and ensure proper aseptic techniques.
Applications of Tetrathionate Bile-Brilliant Green Broth
This medium is commonly used in:
- Food safety testing for Salmonella contamination
- Water quality analysis in environmental microbiology
- Clinical diagnostics for enteric pathogen detection
- Research studies on bacterial growth and inhibition
Its versatility makes it a staple in laboratories across India, from academic institutions to industrial quality control labs.
Why This Medium Stands Out
Unlike generic enrichment broths, Tetrathionate Bile-Brilliant Green Broth offers superior selectivity due to its unique combination of inhibitors. The inclusion of brilliant green enhances the suppression of coliforms and other non-Salmonella bacteria, reducing false positives. This precision is critical in diagnostic and research settings where accuracy is paramount.
For those searching for where to buy brilliant green bile broth online, eqipped provides a seamless shopping experience with authentic Himedia products.
Storage and Handling
Store the powder in a cool, dry place away from direct sunlight. Once reconstituted, the broth should be used immediately or stored at 2-8°C for short-term use. Always check for signs of contamination before use.
Safety Considerations
This product is for laboratory use only. Handle with care, wearing appropriate personal protective equipment (PPE) such as gloves and lab coats. Avoid inhalation or ingestion. In case of contact with skin or eyes, rinse thoroughly with water.
FAQs
What is Tetrathionate Bile-Brilliant Green Broth used for?
It is used for the selective enrichment of Salmonella species in food, water, and clinical samples.
How do I prepare the broth from the powder?
Dissolve the powder in distilled water, sterilize by autoclaving, and cool before use.
Can this medium detect other bacteria besides Salmonella?
No, it is specifically formulated to inhibit non-Salmonella bacteria, ensuring selective growth.
What is the shelf life of the powder?
The powder has a long shelf life when stored properly, typically 2-3 years from the date of manufacture.
Where can I buy Tetrathionate Bile-Brilliant Green Broth online in India?
You can purchase it from eqipped, a trusted supplier of lab supplies for students, researchers, and educators.
