Description
Specifications Table
| Product Name | Buffered Azide Glucose Glycerol Broth Base |
|---|---|
| Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory/Microbiology Grade |
| Application | Selective enrichment of enterococci and streptococci |
Product Description
Buffered Azide Glucose Glycerol Broth Base (M220) is a specialized microbiology medium designed for the selective enrichment of enterococci and streptococci. This broth base contains sodium azide, which inhibits the growth of Gram-negative bacteria while allowing the targeted organisms to thrive. The buffered formulation ensures pH stability, making it ideal for consistent and reliable results in laboratory settings.
Whether you’re a student conducting experiments or a researcher working on bacterial isolation, this medium provides the necessary nutrients and selective agents to support accurate microbial analysis. The 500 gm pack size is perfect for labs with moderate to high usage, ensuring you have an ample supply for multiple tests.
At eqipped, we understand the importance of quality in microbiology media. That’s why we offer this Himedia product, known for its consistency and performance in bacterial culture applications.
Key Applications
This broth base is commonly used in:
- Clinical microbiology labs for isolating pathogenic bacteria
- Research studies involving enterococci and streptococci
- Quality control testing in food and pharmaceutical industries
- Educational settings for student training in microbiology techniques
Need a reliable medium for your bacterial culture experiments? Buy Buffered Azide Glucose Glycerol Broth Base online today at eqipped for fast delivery and competitive pricing.
How to Use
To prepare the broth, follow these steps:
- Dissolve 50 grams of the powder in 1 liter of distilled water.
- Heat gently while stirring to ensure complete dissolution.
- Autoclave at 121°C for 15 minutes to sterilize.
- Cool to room temperature before inoculation.
For best results, always follow standard microbiological practices and maintain sterile conditions during preparation and use.
Storage and Handling
Store the powder in a cool, dry place away from direct sunlight. Once prepared, the broth should be used within a reasonable timeframe to maintain its selectivity and effectiveness. Always check for signs of contamination before use.
Safety Information
While this product is generally safe for laboratory use, it’s important to handle all microbiological media with care. Wear appropriate personal protective equipment (PPE) such as gloves and lab coats when preparing and using the broth. Dispose of used media according to your institution’s biohazard waste protocols.
FAQs
What is Buffered Azide Glucose Glycerol Broth Base used for?
This broth is specifically designed for the selective enrichment of enterococci and streptococci in microbiological samples. It’s commonly used in clinical labs, research settings, and educational institutions.
How does the sodium azide in this broth work?
Sodium azide acts as a selective agent that inhibits the growth of Gram-negative bacteria while allowing the targeted Gram-positive organisms to grow, making it easier to isolate specific bacteria.
Can I use this broth for environmental samples?
Yes, this medium can be used for environmental samples where enterococci or streptococci isolation is required, though you may need to adjust the sample preparation based on your specific application.
What’s the shelf life of the powder form?
The powder has a long shelf life when stored properly in a cool, dry place. Typically, it remains stable for 2-3 years from the date of manufacture.
Does eqipped offer bulk discounts on this product?
Yes, we offer competitive pricing on bulk orders. Contact our customer support team for information on bulk discounts and institutional pricing.
