Description
Specifications Table
| Product Name | C.L.E.D. HiCynth Agar with Bromo Thymol Blue | MCD792-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Microbiological culture media for bacterial differentiation |
Product Description
When it comes to microbiological research, having the right culture media is crucial for accurate and reliable results. C.L.E.D. HiCynth Agar with Bromo Thymol Blue is a specialized medium designed for the differentiation and identification of bacteria, particularly in urinary tract infection studies. This high-quality agar from Himedia is trusted by researchers and educators across India for its consistency and performance.
C.L.E.D. (Cystine-Lactose-Electrolyte-Deficient) Agar is formulated to support the growth of a wide range of bacteria while inhibiting the swarming of Proteus species. The addition of Bromo Thymol Blue as a pH indicator helps in the differentiation of lactose-fermenting and non-lactose-fermenting bacteria, making it an essential tool in clinical and research laboratories.
Whether you’re a student working on a microbiology project or a researcher conducting advanced studies, this agar provides the clarity and precision needed for your experiments. The 500 gm pack size ensures you have an ample supply for multiple tests, making it a cost-effective choice for labs of all sizes.
Need reliable microbiology media for your lab? Buy C.L.E.D. HiCynth Agar with Bromo Thymol Blue online from eqipped and get fast delivery across India.
Key Applications of C.L.E.D. HiCynth Agar
This versatile agar medium is widely used in various microbiological applications, including:
Urinary Tract Infection (UTI) Diagnosis: C.L.E.D. Agar is commonly used in clinical laboratories to isolate and identify bacteria responsible for urinary tract infections. The medium’s unique formulation helps in distinguishing between different bacterial species, aiding in accurate diagnosis and treatment.
Bacterial Differentiation: The presence of Bromo Thymol Blue allows for easy differentiation between lactose-fermenting and non-lactose-fermenting bacteria. This is particularly useful in identifying Enterobacteriaceae, a family of bacteria that includes common pathogens like E. coli.
Research and Education: For students and educators, C.L.E.D. Agar serves as an excellent tool for teaching microbiological techniques. Its clear differentiation capabilities make it ideal for demonstrating bacterial growth and identification in laboratory settings.
Quality Control: In pharmaceutical and food industries, this agar is used for quality control testing to ensure products are free from harmful bacterial contamination.
Why C.L.E.D. HiCynth Agar with Bromo Thymol Blue Stands Out
Not all culture media are created equal. What sets C.L.E.D. HiCynth Agar apart is its carefully balanced formulation that ensures optimal bacterial growth while providing clear differentiation. The inclusion of Bromo Thymol Blue enhances the medium’s ability to distinguish between different bacterial species, making it a preferred choice for many microbiologists.
The agar’s electrolyte-deficient composition prevents the swarming of Proteus species, which can often interfere with the isolation and identification of other bacteria. This feature is particularly valuable in clinical settings where accurate identification is critical for patient care.
Additionally, the high-quality ingredients used in this Himedia product ensure consistency and reliability in every batch. Whether you’re conducting routine tests or advanced research, you can trust this agar to deliver precise results every time.
How to Use C.L.E.D. HiCynth Agar
Using C.L.E.D. HiCynth Agar is straightforward, but following the correct procedure ensures the best results. Here’s a simple guide to help you get started:
Preparation: Suspend 45 grams of the agar powder in 1 liter of distilled water. Heat gently while stirring to dissolve the medium completely. Avoid overheating to prevent degradation of the nutrients.
Sterilization: Autoclave the prepared medium at 121°C for 15 minutes. Ensure proper sterilization to eliminate any contaminants that could interfere with your results.
Pouring Plates: After sterilization, allow the medium to cool to about 45-50°C. Pour the medium into sterile Petri dishes and let it solidify. The plates are now ready for inoculation.
Inoculation: Using a sterile loop or swab, inoculate the agar plates with your bacterial sample. Spread the sample evenly across the surface of the agar.
Incubation: Incubate the plates at 35-37°C for 18-24 hours. After incubation, observe the bacterial growth and note the color changes due to the Bromo Thymol Blue indicator.
Interpretation: Lactose-fermenting bacteria will produce yellow colonies due to acid production, while non-lactose-fermenters will appear as blue or colorless colonies. This differentiation is key to identifying the bacterial species present.
Safety and Handling
While C.L.E.D. HiCynth Agar is generally safe to handle, it’s important to follow standard laboratory safety protocols. Always wear appropriate personal protective equipment (PPE), such as gloves and lab coats, when preparing and handling the medium. Ensure proper disposal of used plates and contaminated materials to prevent any potential hazards.
Store the agar powder in a cool, dry place away from direct sunlight. Once prepared, the agar plates should be stored at 2-8°C and used within a reasonable time frame to maintain their efficacy.
Where to Buy C.L.E.D. HiCynth Agar with Bromo Thymol Blue
Finding high-quality lab supplies can be challenging, but eqipped makes it easy. As a trusted supplier of laboratory products in India, eqipped offers a wide range of microbiology media, including C.L.E.D. HiCynth Agar with Bromo Thymol Blue. With fast shipping and reliable customer service, you can rest assured that your order will arrive on time and in perfect condition.
Looking for a reliable supplier of microbiology media? Shop C.L.E.D. HiCynth Agar with Bromo Thymol Blue online at eqipped and experience hassle-free delivery.
FAQs
What is C.L.E.D. HiCynth Agar used for?
C.L.E.D. HiCynth Agar is primarily used for the differentiation and identification of bacteria, especially in urinary tract infection studies. It helps distinguish between lactose-fermenting and non-lactose-fermenting bacteria.
How does Bromo Thymol Blue help in bacterial differentiation?
Bromo Thymol Blue acts as a pH indicator. Lactose-fermenting bacteria produce acid, turning the medium yellow, while non-lactose-fermenters do not change the color, making it easier to identify different bacterial species.
Can I use C.L.E.D. HiCynth Agar for educational purposes?
Yes, this agar is excellent for educational settings. Its clear differentiation capabilities make it ideal for teaching microbiological techniques and bacterial identification in labs.
What is the shelf life of C.L.E.D. HiCynth Agar?
The agar powder has a long shelf life when stored properly in a cool, dry place. Once prepared, the agar plates should be used within a few weeks for best results.
How do I store C.L.E.D. HiCynth Agar?
Store the agar powder in a cool, dry place away from direct sunlight. Prepared agar plates should be refrigerated at 2-8°C and used within a reasonable time frame.
