Description
Specifications Table
| Product Name | CHAPSO 96% For Biochemistry |
|---|---|
| Pack Size | 1 g, 5 g |
| Form | Solid |
| Grade | Laboratory Grade |
| Application | Protein solubilization, membrane research, biochemical assays |
Product Description
CHAPSO 96% is a high-purity zwitterionic detergent widely used in biochemistry and molecular biology research. As a derivative of CHAPS, it offers excellent solubility properties for membrane proteins while maintaining their native structure. This makes it an essential reagent for protein purification, crystallization, and functional studies.
Researchers and students working in biochemistry labs often rely on CHAPSO for its gentle yet effective solubilization capabilities. Unlike harsher detergents, CHAPSO preserves protein activity, making it ideal for enzyme assays and structural biology applications. Its compatibility with various buffer systems further enhances its versatility in experimental setups.
When you buy CHAPSO 96% online from eqipped, you get a product that meets stringent quality standards, ensuring reproducibility in your experiments. Whether you’re working on membrane protein extraction or studying protein-lipid interactions, this laboratory-grade detergent delivers consistent results.
Need reliable CHAPSO 96% for your research? Order now and experience seamless delivery across India.
FAQs
What is the difference between CHAPSO and CHAPS?
CHAPSO is the sulfobetaine derivative of CHAPS, offering similar properties but with slightly different solubility characteristics. CHAPSO is often preferred for its higher critical micelle concentration and different hydrophilic-lipophilic balance.
Can CHAPSO be used for cell lysis?
Yes, CHAPSO is effective for gentle cell lysis, particularly when preserving protein structure is important. It’s commonly used in combination with other reagents for optimal cell disruption.
What concentration of CHAPSO should I use for protein solubilization?
Typical working concentrations range from 0.1% to 1% (w/v), but optimal concentration depends on your specific protein and application. Always perform titration experiments to determine the best concentration for your needs.
Is CHAPSO compatible with mass spectrometry?
CHAPSO can interfere with mass spectrometry due to its detergent properties. For MS applications, consider using MS-compatible detergents or thorough sample cleanup procedures to remove CHAPSO before analysis.
How should I prepare a stock solution of CHAPSO?
To prepare a 10% (w/v) stock solution, dissolve 1 g of CHAPSO in 10 ml of appropriate buffer (e.g., PBS or Tris buffer). Warm gently if needed, but avoid excessive heat. Filter sterilize if required for your application.
