Description
Specifications Table
Product Name – CHLOROFORM 99.8% AR
Quantity/Pack Size – 500 ml, 1000 ml, 2500 ml
Form – Liquid
Grade – AR (Analytical Reagent)
Application – Solvent extraction, HPLC, UV spectroscopy, organic synthesis
Product Overview
CHLOROFORM 99.8% AR is a high-purity, analytical-grade solvent designed for critical laboratory applications where precision and consistency are paramount. This ultra-refined chloroform undergoes rigorous purification to eliminate impurities, ensuring minimal moisture content (<0.05%) and exceptional batch-to-batch reproducibility. Its 99.8% assay purity makes it particularly suitable for sensitive techniques like HPLC, UV-Vis spectroscopy, and NMR sample preparation, where even trace contaminants could compromise results. The reagent is stabilized with ethanol (0.5-1%) to prevent phosgene formation during storage, maintaining its integrity over extended periods. Packaged in amber glass bottles to prevent photodegradation, this AR-grade chloroform meets stringent international standards (ACS/ISO compliant) for analytical work. Its low residual acidity and non-volatile nature further enhance its reliability in quantitative analyses. The solvent’s excellent miscibility with most organic compounds while being immiscible with water allows for efficient liquid-liquid extractions in organic synthesis protocols.
FAQs
1. What is the shelf life of 99.8% AR chloroform when stored properly?
When stored in tightly sealed original containers at 15-25°C away from direct sunlight, the shelf life is typically 24 months from the date of manufacture. The ethanol stabilizer helps maintain purity during this period.
2. Can this chloroform be used for DNA extraction protocols?
While it meets purity requirements for many molecular biology applications, we recommend verifying compatibility with your specific protocol as some DNA extraction methods may require chloroform with different stabilization additives.
3. What precautions should be taken when handling this AR-grade chloroform?
Always use in a well-ventilated fume hood, wear appropriate PPE (nitrile gloves, safety goggles), and avoid inhalation. The product should be handled in a cold room if working with large volumes to minimize vapor formation.
4. How does the ethanol stabilization affect the solvent’s properties?
The 0.5-1% ethanol acts as a stabilizer to prevent phosgene formation but doesn’t significantly alter the solvent’s polarity or extraction efficiency. It may slightly increase the solvent’s miscibility with water (about 0.8% at 20°C).
5. What’s the typical evaporation rate compared to other common solvents?
Chloroform evaporates faster than water but slower than solvents like acetone or ether. At room temperature (25°C), it has an evaporation rate approximately 5 times that of n-butyl acetate, making it suitable for controlled evaporation procedures.
