Description
Specifications Table
| Product Name | Chloroform: Isoamyl Alcohol (24:1) for Molecular Biology |
|---|---|
| Pack Size | 100 ml, 500 ml |
| Form | Liquid |
| Grade | Molecular Biology |
| Application | DNA/RNA extraction, nucleic acid purification |
Product Description
When it comes to molecular biology research, the quality of reagents can make or break your experiments. Chloroform: Isoamyl Alcohol (24:1) is a critical solvent blend designed specifically for DNA and RNA extraction protocols. This high-purity mixture ensures efficient phase separation during nucleic acid isolation, minimizing contamination and maximizing yield.
Whether you’re a student working on a thesis project or a researcher optimizing a new extraction method, this molecular biology-grade solution provides the consistency and reliability needed for reproducible results. The 24:1 ratio of chloroform to isoamyl alcohol is optimized to prevent emulsion formation, making it easier to recover pure nucleic acids from your samples.
At eqipped, we understand the importance of having dependable lab supplies. That’s why we source this product from SRL Chem, a trusted name in laboratory chemicals, to ensure you receive a product that meets the strictest quality standards for molecular biology applications.
Need a reliable solvent for your DNA extraction workflow? Order Chloroform: Isoamyl Alcohol (24:1) today and experience the difference quality makes.
Why This Product Stands Out
Not all chloroform blends are created equal. This molecular biology-grade solution is specially formulated to:
- Enhance phase separation during nucleic acid extraction
- Reduce the risk of sample contamination
- Improve the purity of isolated DNA/RNA
- Maintain stability during storage and use
The 24:1 ratio is particularly effective in preventing the formation of stubborn emulsions that can complicate the extraction process. This means less time spent troubleshooting and more time focusing on your research goals.
Applications in the Lab
This versatile solvent blend is commonly used in:
- Phenol-chloroform extraction of DNA/RNA
- Plasmid DNA isolation protocols
- Genomic DNA purification from tissue samples
- RNA extraction for RT-PCR applications
For students learning molecular biology techniques, this product provides a consistent baseline for experiments, helping to eliminate variables that could affect results. Researchers will appreciate the reproducibility it brings to their workflows.
Safety and Handling
While chloroform: isoamyl alcohol is an essential lab reagent, it requires careful handling. Always work in a well-ventilated area or fume hood, and use appropriate personal protective equipment including gloves and safety goggles. Store the bottle tightly sealed in a cool, dry place away from direct sunlight.
Remember that chloroform is a hazardous chemical. Avoid inhalation and skin contact. In case of accidental exposure, follow standard laboratory safety protocols and consult the material safety data sheet (MSDS) for specific first aid measures.
Choosing the Right Pack Size
We offer this product in two convenient sizes to match your lab’s needs:
- 100 ml – Ideal for small-scale experiments or occasional use
- 500 ml – Perfect for high-throughput labs or frequent extractions
For teaching labs or research facilities with varying needs, the 500 ml size provides better value while ensuring you always have this critical reagent on hand when needed.
Quality You Can Trust
Every batch of Chloroform: Isoamyl Alcohol (24:1) from SRL Chem undergoes rigorous quality control to ensure it meets molecular biology grade specifications. This means you can trust that each bottle delivers the same high performance, experiment after experiment.
At eqipped, we’re committed to providing researchers and students with lab supplies that meet the highest standards. That’s why we’ve made this essential molecular biology reagent available through our platform, with the convenience of online ordering and reliable delivery.
FAQs
What is the purpose of isoamyl alcohol in this mixture?
Isoamyl alcohol is added to chloroform to prevent emulsion formation during nucleic acid extraction. The 24:1 ratio is optimized to create a clear phase separation between the aqueous and organic layers, making it easier to isolate pure DNA or RNA.
Can I use this product for protein extraction?
While this blend is primarily designed for nucleic acid extraction, it can be used in some protein purification protocols. However, we recommend consulting your specific protocol requirements as different applications may need adjusted ratios or additional reagents.
How should I store this product?
Store the bottle tightly sealed in a cool, dry place away from direct sunlight and heat sources. For best results, keep it in a chemical storage cabinet designated for flammable liquids. Always ensure the cap is properly closed after each use to prevent evaporation.
What safety precautions should I take when using this product?
Always work in a fume hood or well-ventilated area. Wear appropriate PPE including nitrile gloves and safety goggles. Avoid inhalation and skin contact. In case of spills, clean immediately using proper laboratory procedures for organic solvent spills.
Is this product suitable for undergraduate lab courses?
Yes, this molecular biology grade chloroform: isoamyl alcohol blend is excellent for teaching labs. Its consistent quality helps students achieve reliable results in DNA extraction experiments, making it easier to demonstrate fundamental molecular biology techniques.
