Description
Specifications Table
Product Name – Collagenase Purified
Quantity/Pack Size – 10000 units
Form – Lyophilized Powder
Grade – Purified
Application – Tissue Dissociation, Cell Isolation, Extracellular Matrix Studies
Product Overview
Collagenase Purified is a highly specialized enzyme, meticulously processed to ensure optimal activity and purity for demanding laboratory applications. Derived from Clostridium histolyticum, this powerful enzyme is renowned for its ability to specifically hydrolyze native and denatured collagen, a critical component of the extracellular matrix. Its targeted action makes it indispensable for researchers requiring efficient and gentle tissue dissociation, enabling the isolation of various cell types from complex tissues. This purified grade ensures minimal contamination from other proteolytic enzymes, thereby enhancing cell viability and experimental reproducibility. Ideal for preparing primary cell cultures, conducting studies in tissue engineering, regenerative medicine, and wound healing research, our Collagenase Purified offers consistent performance. Its reliable enzymatic activity ensures dependable results across a broad spectrum of biological investigations, making it a cornerstone reagent for advanced cellular and molecular biology laboratories. Researchers can trust this product for its high quality and consistent performance, facilitating groundbreaking discoveries.
FAQs
1. What is the primary function of Collagenase Purified?
Collagenase Purified is an enzyme specifically designed to break down collagen, a major component of extracellular matrix, facilitating tissue dissociation and the isolation of various cell types for research.
2. How should Collagenase Purified be stored to maintain its activity?
For optimal stability and enzymatic activity, Collagenase Purified should be stored lyophilized at -20°C. Reconstituted solutions should be used immediately or stored briefly at 4°C.
3. Is this enzyme compatible with all cell types for isolation?
While Collagenase Purified is widely used, its efficacy can vary depending on tissue source and cell type. It is generally compatible with many mammalian cells, but optimization may be required for specific applications.
4. Are there alternatives to Collagenase Purified for tissue dissociation?
Yes, other enzymes like Trypsin, Dispase, or Papain can be used, often in combination. Collagenase Purified is distinct for its specific collagen degradation, crucial when collagen breakdown is the primary objective.
5. What is the recommended method for reconstituting Collagenase Purified?
It is typically reconstituted in a sterile buffer solution, such as Hank’s Balanced Salt Solution (HBSS) or Dulbecco’s Modified Eagle Medium (DMEM), free of calcium, to the desired working concentration just prior to use.
