Description
Specifications Table
| Product Name | Collagenase Purified | TC1253 | CDH |
|---|---|
| Quantity/Pack Size | 100 mg |
| Form | Lyophilized Powder |
| Grade | Research Grade |
| Application | Tissue dissociation, cell isolation, enzymatic digestion |
Product Description
Collagenase Purified (TC1253) is a highly refined enzyme designed for precise tissue dissociation in research applications. Sourced from Clostridium histolyticum, this collagenase offers superior activity with minimal non-specific proteolysis, ensuring gentle yet effective cell isolation. Whether you’re working with mammalian tissues, primary cell cultures, or complex matrices, this enzyme delivers consistent results with reduced batch-to-batch variability.
Researchers across India trust eqipped for reliable lab supplies, and this collagenase is no exception. Its purified formulation minimizes contaminants, making it ideal for sensitive applications like stem cell research, tumor dissociation, and organoid culture. The lyophilized powder format ensures long-term stability, while the research-grade purity meets the stringent demands of modern laboratories.
Need high-quality collagenase for your next experiment? Buy Collagenase Purified online today and experience the difference in your tissue dissociation protocols.
One of the key advantages of this collagenase is its balanced activity profile. Unlike crude collagenase blends, this purified version targets collagen fibers specifically, preserving cell viability and surface markers. This makes it particularly valuable for downstream applications like flow cytometry, single-cell sequencing, or functional assays where cell integrity is critical.
For best results, reconstitute the lyophilized powder in sterile buffer (typically HBSS or PBS with calcium) and use immediately. The enzyme remains active at physiological pH and temperature, though optimal conditions may vary based on tissue type. Always perform titration experiments to determine the ideal concentration for your specific application.
Applications in Research
This collagenase finds extensive use in:
- Isolating primary cells from tissues like liver, adipose, or pancreatic samples
- Dissociating tumor tissues for cancer research
- Preparing single-cell suspensions for flow cytometry
- Generating organoids and 3D cell cultures
- Neuroscience research involving brain tissue dissociation
Many Indian research labs prefer this collagenase for its reliability in protocols requiring gentle yet thorough tissue breakdown. The purified nature reduces the need for additional purification steps, saving valuable time in experimental workflows.
Storage and Handling
Store the lyophilized powder at -20°C for long-term stability. Once reconstituted, aliquot and store at -20°C for up to 6 months or at 4°C for short-term use (1-2 weeks). Avoid repeated freeze-thaw cycles to maintain enzyme activity.
Always wear appropriate personal protective equipment when handling. While this is a purified preparation, treat it as a potential biohazard and follow standard laboratory safety protocols.
Why Researchers Choose This Collagenase
In the competitive landscape of research enzymes, this collagenase stands out for several reasons:
- Consistent lot-to-lot performance, crucial for reproducible results
- Minimal non-specific protease activity compared to crude preparations
- High specific activity, reducing the amount needed per experiment
- Low endotoxin levels, suitable for sensitive cell cultures
- Backed by eqipped’s commitment to quality lab supplies
Ready to upgrade your tissue dissociation protocol? Order Collagenase Purified now and get it delivered to your lab in India within days.
Comparing Collagenase Types
Not all collagenases are created equal. This purified version offers distinct advantages over:
- Crude collagenase: Higher specificity, fewer contaminants
- Type I/II blends: More consistent activity profiles
- Recombinant collagenases: Often more cost-effective for routine use
For applications requiring the highest purity, consider supplementing with DNase or other proteases as needed for your specific tissue type.
FAQs
What is the ideal working concentration for Collagenase Purified?
Typical working concentrations range from 0.1-2 mg/mL, but optimal concentration depends on tissue type. For soft tissues like adipose, 0.5-1 mg/mL often works well, while denser tissues may require up to 2 mg/mL. Always perform titration experiments for your specific application.
Can this collagenase be used for human tissue samples?
Yes, this research-grade collagenase is suitable for human tissue dissociation. However, ensure your institution’s ethical guidelines and biosafety protocols are followed when working with human samples.
How does this compare to collagenase from other suppliers?
This purified collagenase offers competitive specific activity with lower batch variability compared to many alternatives. The purification process removes many contaminants found in crude preparations, making it gentler on cells while maintaining effective dissociation.
What buffer should I use for reconstitution?
We recommend reconstituting in HBSS (Hank’s Balanced Salt Solution) with calcium and magnesium. Alternatively, PBS with 1-2 mM calcium chloride works well. Avoid buffers with chelators like EDTA as they inhibit collagenase activity.
Is this collagenase suitable for GMP applications?
This is a research-grade product not manufactured under GMP conditions. For clinical or therapeutic applications, please contact us for information about our GMP-compliant alternatives.
