Description
Specifications Table
Product Name – Coomassie Brilliant Blue R 250 C.I. 42660 HISURE
Quantity/Pack Size – 5 gm
Form – Powder
Grade – Laboratory Grade
Application – Protein Staining, SDS-PAGE, Electrophoresis
Product Overview
Coomassie Brilliant Blue R 250 C.I. 42660 HISURE is a premium laboratory dye, widely recognized for its exceptional effectiveness in various biochemical applications, particularly protein staining. This highly purified reagent delivers consistent and reliable results, making it an indispensable tool for researchers and educators demanding precision. As a classic triphenylmethane dye, R-250 exhibits a characteristic intense blue color upon binding to proteins, facilitating clear and unambiguous visualization and subsequent analysis. Its interaction with basic and aromatic amino acid residues, primarily through electrostatic and hydrophobic forces, enables robust and sensitive detection of proteins across a wide concentration range, establishing it as a cornerstone reagent.
Manufactured to stringent quality standards, HISURE’s Coomassie Brilliant Blue R 250 ensures minimal impurities, which is crucial for achieving reproducible experimental outcomes and maintaining data integrity in sensitive assays. The fine powder form allows for precise and easy preparation of staining solutions, offering significant flexibility in concentration adjustments for various experimental protocols. This widely recognized dye is extensively employed for post-electrophoretic visualization of protein bands, consistently providing sharp, distinct staining that is easily quantifiable and photographable. It offers a cost-effective and straightforward method for general protein detection, serving as a foundational reagent in molecular biology, biochemistry, and proteomics laboratories globally.
The inherent reliability of Coomassie Brilliant Blue R 250 extends to its excellent stability and ease of handling, ensuring a prolonged shelf life and straightforward integration into existing laboratory workflows without complex procedures. Its distinct spectral properties make it suitable for various detection and imaging systems, further enhancing its utility across diverse research settings. Choosing HISURE’s Coomassie Brilliant Blue R 250 guarantees a high-performance, consistent reagent, essential for obtaining accurate and impactful results in protein analysis and related studies, underscoring its pivotal role in contemporary scientific investigation and setting a benchmark for quality.
FAQs
1. What is Coomassie Brilliant Blue R 250 primarily used for?
Coomassie Brilliant Blue R 250 is primarily used as a general protein stain, especially for visualizing protein bands in polyacrylamide gel electrophoresis (SDS-PAGE) and for other protein detection assays.
2. What is the difference between Coomassie R-250 and G-250?
The main difference lies in their sensitivity and applications. R-250 (Reddish) is generally used for staining gels, while G-250 (Greenish) is more commonly used in the Bradford assay for protein quantification due to its higher sensitivity in solution, though R-250 can also be used for gel staining.
3. How should Coomassie Brilliant Blue R 250 be stored?
For optimal stability and shelf life, Coomassie Brilliant Blue R 250 should be stored in a tightly sealed container in a cool, dry place, protected from light. Ensure the storage area maintains consistent conditions.
4. Is this product suitable for quantitative protein analysis?
While R-250 is excellent for visualizing protein bands, for precise quantitative protein analysis in solution (e.g., Bradford assay), Coomassie Brilliant Blue G-250 is generally preferred due to its higher sensitivity. However, stained gels can be quantified using densitometry.
5. What ensures the high quality of HISURE’s Coomassie Brilliant Blue R 250?
HISURE’s Coomassie Brilliant Blue R 250 is manufactured under stringent quality control standards, ensuring high purity and minimal impurities. This rigorous process guarantees consistent performance and reliable results for your laboratory experiments.
