Description
Specifications Table
| Product Name | Cytochalasin B Cell Culture Tested |
|---|---|
| Quantity/Pack Size | 5mg |
| Form | Solid |
| Grade | Cell Culture Tested |
| Application | Actin filament disruption, cell biology research |
Product Description
Cytochalasin B is a potent mycotoxin widely used in cell biology to inhibit actin polymerization, making it indispensable for studying cytoskeleton dynamics. This cell culture-tested grade ensures minimal cytotoxicity, allowing researchers to observe cellular processes without unwanted interference. Whether you’re investigating cell motility, cytokinesis, or membrane trafficking, this high-purity reagent delivers consistent results.
At eqipped, we understand the importance of reliable reagents in research. Our Cytochalasin B undergoes rigorous quality checks to meet the demands of modern laboratories. The 5mg pack size is ideal for both small-scale experiments and larger projects, providing flexibility without compromising on purity.
Need Cytochalasin B for your next experiment? Buy online now and experience seamless research with eqipped.
How Cytochalasin B Works in Cell Culture
Cytochalasin B binds to the barbed ends of actin filaments, preventing further elongation. This disruption helps researchers study:
- Cell division and cytokinesis
- Cell migration and adhesion
- Endocytosis and exocytosis pathways
- Nuclear shape and chromatin organization
For best results, dissolve in DMSO or ethanol before adding to cell culture media. Always perform a dose-response assay to determine the optimal concentration for your specific cell line.
Applications in Research
This versatile compound is a staple in laboratories working on:
- Cancer research (studying metastasis and invasion)
- Neuroscience (analyzing neuronal growth cones)
- Immunology (investigating immune cell motility)
- Developmental biology (observing embryonic morphogenesis)
Looking for a trusted cytochalasin b supplier? eqipped offers lab-grade reagents with fast shipping across India.
Safety and Handling
Cytochalasin B is a hazardous substance. Follow these precautions:
- Wear appropriate PPE (gloves, lab coat, goggles)
- Handle in a fume hood to avoid inhalation
- Store at -20°C, protected from light
- Dispose of waste according to local regulations
Always refer to the Safety Data Sheet (SDS) before use. Not for human or veterinary use.
FAQs
What is the solubility of Cytochalasin B?
Cytochalasin B is soluble in DMSO (up to 10 mg/mL) and ethanol. For cell culture applications, prepare a stock solution in DMSO and dilute further in media.
How should I store Cytochalasin B?
Store the solid at -20°C, desiccated, and protected from light. Solutions should be aliquoted and stored at -20°C for up to 6 months.
What concentration of Cytochalasin B should I use?
Typical working concentrations range from 0.5 to 10 µg/mL, depending on the cell type. Always optimize for your specific application.
Is this product suitable for in vivo studies?
No, this grade is specifically tested for in vitro cell culture applications. For in vivo use, consult regulatory guidelines.
How quickly does Cytochalasin B act on cells?
Effects on actin filaments are typically observed within 30 minutes to 2 hours, depending on the concentration and cell type.