Description
Specifications Table
| Product Name | Deoxycholate Citrate Agar Plate |
|---|---|
| Quantity/Pack Size | 10 plates |
| Form | Pre-poured agar plates |
| Grade | Laboratory grade |
| Application | Isolation of Salmonella and Shigella from clinical and food samples |
Product Description
Deoxycholate Citrate Agar Plate | MP065-10PT | Himedia is a ready-to-use selective and differential microbiological culture medium designed primarily for the isolation and differentiation of enteric bacteria, particularly Salmonella and Shigella species, from clinical, food, water, and other microbiological samples. Deoxycholate Citrate Agar (DCA) is formulated to inhibit many Gram-positive organisms and several non-target Gram-negative bacteria while supporting the growth of important enteric pathogens.
The selective properties of the medium are primarily associated with sodium deoxycholate and citrate, which help suppress unwanted microbial flora and improve the recovery of target enteric organisms. The differential properties of the agar are based on carbohydrate fermentation and related indicator reactions, allowing colonies with different metabolic characteristics to be distinguished by their appearance on the plate.
Deoxycholate Citrate Agar is commonly used as part of microbiological screening procedures where the isolation of intestinal pathogens is required. It can be particularly useful for the examination of samples suspected of containing Salmonella or Shigella. The medium can help separate suspected colonies from background organisms, making it easier for laboratory personnel to select colonies for subsequent identification and confirmation.
The MP065-10PT format is supplied as a prepared, ready-to-use agar plate. This eliminates the need for laboratories to weigh individual ingredients, prepare the medium, sterilize it, and pour plates manually. The ready-to-use format can help streamline routine microbiological workflows and reduce variations associated with in-house media preparation.
Deoxycholate Citrate Agar Plates can be suitable for applications in clinical microbiology, food and beverage testing, water analysis, public health laboratories, research facilities, and educational microbiology laboratories. They may be incorporated into a broader workflow involving enrichment, selective plating, colony examination, and confirmatory identification procedures.
After inoculation and incubation under the conditions specified by the applicable method, microbial colonies can be examined for characteristic morphology and reactions. Suspected colonies should be subjected to appropriate biochemical, serological, molecular, or other validated identification procedures because colony appearance on selective media alone is not sufficient for definitive identification.
The prepared plate format supports convenient handling and can be particularly beneficial for laboratories performing regular microbiological testing. Proper storage, aseptic handling, inoculation, incubation, interpretation, and disposal should follow the manufacturer’s instructions and the laboratory’s validated procedures.
Overall, the Himedia Deoxycholate Citrate Agar Plate provides a convenient selective and differential medium for the isolation and preliminary differentiation of enteric bacteria, particularly Salmonella and Shigella, in routine microbiological examination workflows.
FAQs
1. What is Deoxycholate Citrate Agar Plate used for?
Deoxycholate Citrate Agar Plate is primarily used for the selective isolation and preliminary differentiation of enteric bacteria, particularly Salmonella and Shigella species, from various microbiological samples.
2. How does Deoxycholate Citrate Agar provide selectivity?
The medium contains selective components such as sodium deoxycholate and citrate, which help inhibit many unwanted microorganisms and support the recovery of target enteric bacteria.
3. Can this agar confirm Salmonella or Shigella?
No. Deoxycholate Citrate Agar is intended for selective isolation and preliminary differentiation. Suspected colonies should undergo appropriate biochemical, serological, molecular, or other validated confirmatory tests for definitive identification.
4. Is the MP065-10PT plate ready to use?
Yes. The MP065-10PT product is supplied as a prepared, ready-to-use agar plate, reducing the need for in-house media preparation, sterilization, and plate pouring.
5. What is the pack size and product code?
The product code is MP065-10PT, and the product is supplied as a pack of 10 ready-to-use plates manufactured by Himedia.
