Description
Specifications Table
| Product Name | Deoxyribonuclease I – Type 2B (DNase I) ex. Bovine Pancreas for MB |
|---|---|
| Pack Size | 10 Mg, 100 Mg, 1 Gms |
| Form | Lyophilized Powder |
| Grade | Molecular Biology Grade |
| Application | DNA degradation, RNA isolation, plasmid preparation |
Product Description
When precision matters in molecular biology, Deoxyribonuclease I (DNase I) from bovine pancreas stands out as a reliable enzyme for degrading DNA without affecting RNA. This Type 2B variant, with an activity of 180 Kunitz units per milligram and 85% protein purity, is specifically designed for applications where contamination-free RNA extraction or plasmid preparation is critical.
Whether you’re a student setting up your first RNA isolation experiment or a researcher optimizing a protocol, this DNase I ensures consistent performance. Its high specificity for double-stranded and single-stranded DNA makes it indispensable in labs where RNA integrity is non-negotiable. The lyophilized form guarantees stability, allowing you to store it long-term without losing potency.
At eqipped, we understand the frustration of inconsistent reagents derailing experiments. That’s why we source this DNase I from trusted manufacturers, ensuring every batch meets rigorous quality standards. From removing DNA contaminants in RNA preps to nicking DNA for cloning, this enzyme delivers results you can trust.
Need DNase I for your next molecular biology experiment? Buy DNase I enzyme online today and experience the difference quality makes.
How DNase I Works in Molecular Biology
DNase I catalyzes the hydrolysis of phosphodiester bonds in DNA, breaking it down into oligonucleotides. Unlike some nucleases, it doesn’t require divalent cations like Mg²⁺ for activity, making it versatile for various buffer conditions. In RNA isolation workflows, it eliminates genomic DNA contamination that could interfere with downstream applications like RT-PCR or sequencing.
For researchers working with plasmids, DNase I can introduce nicks in supercoiled DNA, aiding in structural studies or cloning. Its broad pH range (5–9) and optimal activity at physiological pH (7–8) make it adaptable to most lab protocols. Just remember: always include EDTA in your storage buffer to inhibit any trace metal-dependent DNase activity.
Applications Where This DNase I Excels
This bovine pancreas-derived DNase I is a workhorse in labs focusing on:
- RNA isolation from tissues or cells (removing DNA contaminants)
- Preparing DNA-free RNA for RT-PCR, qPCR, or NGS
- Plasmid preparation and linearization
- Studying chromatin structure via DNase I hypersensitivity assays
- Degrading DNA in protein purification workflows
Its high Kunitz unit activity (180 U/mg) means you need less enzyme per reaction, reducing costs without compromising efficiency. The 85% protein purity minimizes non-specific interactions, ensuring cleaner results.
Why Molecular Biologists Prefer This DNase I
Not all DNase I enzymes are created equal. This Type 2B variant from bovine pancreas is favored because:
- It’s free from RNase and protease contaminants, protecting your RNA samples.
- The lyophilized format ensures long shelf life—just reconstitute with sterile water when needed.
- Its activity is standardized in Kunitz units, so you can easily calculate dosing for your experiments.
- It’s compatible with common molecular biology buffers, reducing the need for optimization.
Upgrade your lab’s RNA workflows. Shop DNase I for molecular biology now and eliminate DNA contamination for good.
Handling and Storage Tips
To maintain the enzyme’s activity:
- Store the lyophilized powder at -20°C. Once reconstituted, aliquot and store at -20°C for up to 6 months.
- Avoid repeated freeze-thaw cycles, as this can denature the protein.
- Use nuclease-free water or buffers for reconstitution to prevent contamination.
- For RNA isolation, always include an inactivation step (e.g., heat or phenol-chloroform extraction) after DNase treatment.
Safety and Risk Note
While DNase I is generally safe, handle it with standard lab precautions:
- Wear gloves and lab coats to avoid skin contact.
- Work in a fume hood if handling large quantities to prevent inhalation.
- Dispose of waste according to your institution’s biohazard protocols.
FAQs
What is the difference between DNase I Type 2B and other DNase I variants?
Type 2B DNase I from bovine pancreas is highly purified and standardized for molecular biology use. It has a defined Kunitz unit activity (180 U/mg) and is free from RNase/protease contaminants, unlike crude preparations.
How do I calculate the amount of DNase I needed for my RNA isolation?
A typical protocol uses 1–10 Kunitz units per 100 µg of RNA. For example, for 100 µg RNA, use 1–10 µL of a 1 U/µL solution. Always optimize based on your sample type.
Can this DNase I be used for chromatin accessibility assays?
Yes, DNase I hypersensitivity assays rely on this enzyme to cleave accessible chromatin regions. Its high purity ensures minimal background noise in such applications.
Is this DNase I compatible with my RNA extraction kit?
Most commercial RNA extraction kits include a DNase I treatment step. This enzyme can be used as a standalone or supplementary DNase, but check your kit’s protocol for compatibility.
How do I inactivate DNase I after RNA treatment?
Common methods include heat inactivation (65°C for 10 minutes) or phenol-chloroform extraction. Some kits provide DNase inactivation reagents for convenience.
