Description
Specifications Table
| Product Name | Dextrose Mannitol Agar (Gillies Agar No.1) |
|---|---|
| Pack Size | 100 Gms, 500 Gms |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Microbial testing, staphylococci isolation |
Product Description
Dextrose Mannitol Agar, also known as Gillies Agar No.1, is a specialized microbiological medium designed for the selective isolation and identification of pathogenic staphylococci. This agar contains mannitol as the primary carbohydrate source, which allows for the differentiation of Staphylococcus aureus from other staphylococci based on mannitol fermentation.
The medium is enriched with dextrose to support the growth of fastidious organisms while inhibiting the growth of certain gram-negative bacteria. Its unique formulation makes it an essential tool in clinical and research laboratories for diagnosing infections and studying bacterial behavior.
Whether you’re a student conducting experiments or a researcher working on microbial studies, this agar provides reliable results. eqipped ensures you get high-quality lab supplies delivered to your doorstep, so you can focus on your work without worrying about procurement.
Key Applications
Dextrose Mannitol Agar is widely used in:
- Clinical microbiology for identifying Staphylococcus aureus
- Research laboratories studying bacterial metabolism
- Educational institutions for teaching microbiological techniques
- Food and pharmaceutical industries for quality control testing
Why This Agar Stands Out
Unlike generic agar formulations, Dextrose Mannitol Agar offers superior selectivity due to its balanced composition. The inclusion of mannitol allows for clear differentiation of pathogenic strains, while the dextrose ensures robust growth of target organisms. This makes it a preferred choice for professionals who need accurate and reproducible results.
When you buy Dextrose Mannitol Agar online from eqipped, you’re choosing a product that meets stringent quality standards. Our supplies are sourced from trusted manufacturers, ensuring consistency in every batch.
How to Use
Preparing Dextrose Mannitol Agar is straightforward. Suspend the powder in distilled water, heat to boiling to dissolve completely, and sterilize by autoclaving. Once cooled, pour into sterile Petri dishes and allow to solidify. Inoculate with your sample and incubate at the appropriate temperature for optimal results.
For best practices, always follow standard microbiological procedures to avoid contamination. Store the prepared agar plates at 2-8°C and use within the recommended shelf life.
Safety and Handling
While Dextrose Mannitol Agar is generally safe for laboratory use, it’s important to handle all microbiological media with care. Wear appropriate personal protective equipment (PPE) such as gloves and lab coats when preparing or handling the agar. Dispose of used plates and materials according to your institution’s biohazard waste protocols.
FAQs
What is the shelf life of Dextrose Mannitol Agar?
The powdered medium typically has a shelf life of 2-3 years when stored in a cool, dry place. Prepared plates should be used within a few weeks for best results.
Can this agar be used for environmental sampling?
Yes, Dextrose Mannitol Agar is suitable for environmental sampling, especially when screening for staphylococcal contamination in food processing or healthcare settings.
How does mannitol fermentation help in identification?
Staphylococcus aureus ferments mannitol, producing acid that turns the phenol red indicator yellow. Non-fermenting strains leave the medium unchanged, aiding in differentiation.
Is this product available in ready-to-use plates?
Currently, eqipped offers Dextrose Mannitol Agar in powder form. However, you can easily prepare plates in your lab following standard protocols.
What is the ideal incubation temperature for this agar?
For optimal results, incubate inoculated plates at 35-37°C for 24-48 hours. This temperature range supports the growth of most staphylococcal species.
