Description
Specifications Table
Product Name – Dichloromethane, 99.9%, for biochemistry, stabilized with approx. 50 ppm amylene, AcroSeal®
Quantity/Pack Size – 1LT, 2.5LT
Form – Liquid
Grade – 99.9% (HPLC/Biochemistry Grade)
Application – Solvent extraction, chromatography, protein sequencing, tissue processing
Product Overview
This high-purity 99.9% dichloromethane (DCM) is specifically formulated for demanding biochemistry applications, featuring stabilization with approximately 50 ppm amylene to prevent phosgene formation during storage. The AcroSeal® packaging system maintains solvent integrity by minimizing moisture absorption and evaporation, ensuring consistent performance in sensitive laboratory procedures. With its low UV absorbance and residual impurity profile, this DCM grade excels in HPLC applications, protein sequencing protocols, and lipid extraction workflows where solvent purity directly impacts analytical accuracy. The stabilized formulation extends shelf life while preserving the solvent’s characteristic properties: a boiling point of 39.6°C, density of 1.325 g/mL at 20°C, and miscibility with most organic solvents. The product undergoes rigorous quality control to meet ACS reagent specifications, with certified limits for water content (<0.01%), non-volatile residues (<5 ppm), and heavy metal impurities. The amber glass bottles with PTFE-lined caps prevent light-induced degradation while maintaining chemical resistance, making this an ideal choice for laboratories requiring reproducible results in organic synthesis and analytical chemistry applications.
FAQs
1. What is the significance of amylene stabilization in this dichloromethane?
The 50 ppm amylene acts as a sacrificial stabilizer that reacts with any trace HCl or phosgene formed during storage, preventing the accumulation of these corrosive byproducts that could interfere with sensitive biochemical reactions or damage equipment.
2. Can this DCM be used for tissue processing in histology?
Yes, the high purity and low water content make it suitable for tissue clearing and dehydration steps in histology protocols, though you should verify compatibility with your specific staining procedures as some dyes may be solvent-sensitive.
3. How does the AcroSeal® packaging differ from standard solvent bottles?
The AcroSeal® system incorporates a specialized septum and valve design that creates a vapor-tight seal, reducing solvent loss through evaporation by up to 90% compared to traditional screw-cap bottles while preventing atmospheric moisture ingress.
4. What precautions should be taken when storing this stabilized DCM?
Store at 15-25°C in a dedicated flammable solvent cabinet away from oxidizing agents and direct sunlight. The stabilized formulation has a 24-month shelf life when unopened, but always check for cloudiness or color changes before use as indicators of degradation.
5. Is this grade compatible with mass spectrometry applications?
While suitable for many LC-MS applications due to its low impurity profile, you may need additional purification steps for ultra-trace analysis as the amylene stabilizer could potentially appear in background spectra at very low concentrations.
