Description
Specifications Table
| Product Name | Diluting Fluid D w/ 1% Tween 80 |
|---|---|
| Pack Size | 10 × 100 ml |
| Form | Liquid |
| Grade | Laboratory Grade |
| Application | Microscopy, Staining, Sample Dilution |
Product Description
When precision matters in microscopy and staining, Diluting Fluid D with 1% Tween 80 from Himedia is your go-to solution. Designed for researchers, students, and lab professionals, this high-quality diluting fluid ensures consistent and reliable results in sample preparation. Whether you’re working on bacterial smears, cellular studies, or diagnostic staining, this fluid maintains sample integrity while preventing clumping or aggregation.
The addition of 1% Tween 80, a non-ionic surfactant, enhances the wetting properties of the fluid, ensuring even distribution of samples on slides. This is particularly useful in immunological assays, Gram staining, and other microscopic techniques where uniformity is critical. The 100 ml bottles are conveniently packed in sets of 10, making it ideal for labs with frequent usage.
At eqipped, we understand the importance of using the right reagents for accurate diagnostics and research. This diluting fluid is manufactured under strict quality controls, ensuring batch-to-batch consistency. It’s compatible with most standard staining protocols and can be used directly or further diluted as per experimental requirements.
Need a reliable diluting fluid for your lab? Buy Diluting Fluid D w/ 1% Tween 80 online today and experience seamless sample preparation.
Why This Diluting Fluid Stands Out
Not all diluting fluids are created equal. Here’s what makes this product a preferred choice for labs across India:
Optimal Surface Tension Control: The inclusion of Tween 80 reduces surface tension, allowing samples to spread evenly without beading. This is crucial for techniques like immunofluorescence and histochemistry where uneven spreading can lead to misinterpretation.
Stable and Long Shelf Life: The formulation is designed to remain stable under standard lab storage conditions. Each bottle is sealed to prevent contamination, ensuring you get consistent performance until the last drop.
Versatile Applications: From routine microbial staining to advanced cytological studies, this fluid adapts to various protocols. It’s also widely used in serological tests where precise dilution is key to accurate titrations.
Cost-Effective Packaging: The 10 × 100 ml pack size is perfect for labs that go through diluting fluids regularly. It reduces the need for frequent reordering while keeping storage manageable.
How to Use Diluting Fluid D w/ 1% Tween 80
Using this diluting fluid is straightforward, but following best practices ensures optimal results:
For Microscopy: 1. Clean the microscope slide with alcohol and let it dry. 2. Place a small drop of the diluting fluid on the slide. 3. Add your sample (e.g., bacterial culture, cell suspension) to the fluid. 4. Mix gently with a loop or pipette tip. 5. Proceed with staining or direct observation.
For Staining Protocols: – Use as a diluent for primary or secondary antibodies in immunohistochemical staining. – Dilute stains like Giemsa or Wright’s stain as per protocol requirements. – Ensure proper mixing to avoid concentration gradients.
Storage Tips: – Store bottles at room temperature (15–25°C). – Avoid exposure to direct sunlight or heat sources. – Always recap the bottle tightly after use to prevent evaporation or contamination.
Common Applications in Labs
This diluting fluid is a staple in various lab settings, including:
Clinical Diagnostics: Used in hospitals and diagnostic centers for preparing samples for Gram staining, acid-fast staining, and other differential stains.
Research Laboratories: Essential for cytology, histology, and microbiology research where sample dilution and even spreading are critical.
Educational Institutions: A must-have in university and college labs for practical sessions in microbiology, pathology, and biochemistry.
Pharmaceutical Testing: Employed in quality control labs for microbial limit testing and environmental monitoring.
Safety and Handling
While this diluting fluid is generally safe for lab use, it’s important to follow standard laboratory practices:
– Wear appropriate personal protective equipment (PPE) like gloves and lab coats. – Avoid ingestion or contact with eyes. In case of accidental exposure, rinse thoroughly with water. – Dispose of waste fluid as per your institution’s chemical waste disposal guidelines.
Why Labs Trust Himedia
Himedia is a renowned name in laboratory reagents, known for its commitment to quality and innovation. Their products, including this diluting fluid, undergo rigorous testing to meet international standards. When you buy from eqipped, you’re not just getting a product; you’re investing in reliability and accuracy for your experiments.
FAQs
What is the role of Tween 80 in this diluting fluid?
Tween 80 is a non-ionic surfactant that reduces surface tension, ensuring samples spread evenly on slides. This prevents clumping and improves the quality of microscopic observations.
Can this fluid be used for both Gram-positive and Gram-negative bacteria?
Yes, it’s suitable for preparing smears of both Gram-positive and Gram-negative bacteria. It doesn’t interfere with the staining process, allowing for clear differentiation.
Is this diluting fluid autoclavable?
No, this product is ready-to-use and should not be autoclaved. Autoclaving can degrade the Tween 80 and alter the fluid’s properties.
What is the shelf life of Diluting Fluid D w/ 1% Tween 80?
The shelf life is typically 24 months from the date of manufacture when stored under recommended conditions. Always check the label for the exact expiry date.
Can I use this fluid for diluting antibodies in ELISA?
While it’s primarily designed for microscopy and staining, it can be used for diluting antibodies in some protocols. However, for ELISA, it’s best to use a buffer specifically formulated for immunological assays.
