Description
Specifications Table
Product Name – DITHIOTHREITOL 99% Extra Pure
Quantity/Pack Size – 1 g, 5 g, 25 g
Form – Powder
Grade – Extra Pure
Application – Reducing agent for biochemical assays, protein studies, nucleic acid research
Product Overview
Dithiothreitol (DTT) 99% Extra Pure is a highly effective reducing agent widely used in biochemical and molecular biology laboratories. Its strong reducing properties make it ideal for breaking disulfide bonds in proteins, ensuring proper folding and activity. This extra pure grade guarantees minimal impurities, providing reliable results in sensitive applications. DTT is highly soluble in water and maintains stability under standard lab conditions, making it a preferred choice for researchers. Its ability to protect thiol groups from oxidation enhances its utility in experiments involving enzymes, antibodies, and nucleic acids. The powder form allows for precise dosing, ensuring accuracy in experimental protocols. Whether used in protein purification, DNA/RNA extraction, or enzymatic assays, this high-purity DTT delivers consistent performance, reducing variability in experimental outcomes.
FAQs
1. What is the primary function of Dithiothreitol (DTT) in lab experiments?
DTT is primarily used as a reducing agent to cleave disulfide bonds in proteins, preventing oxidation of thiol groups during experiments.
2. Can DTT be used in place of β-mercaptoethanol?
Yes, DTT is often preferred over β-mercaptoethanol due to its lower volatility and stronger reducing power at neutral pH.
3. How should DTT be stored to maintain its stability?
Store DTT in a cool, dry place, preferably at 2-8°C, and protect it from moisture and light to preserve its efficacy.
4. Is this DTT suitable for use in cell culture applications?
While DTT is not typically used in live cell cultures due to toxicity, it is effective in lysing cells for protein extraction.
5. What is the shelf life of DTT 99% Extra Pure?
When stored under recommended conditions, DTT 99% Extra Pure has a shelf life of 2-3 years from the date of manufacture.
