Description
Specifications Table
| Product Name | DL-Dithiothreitol (DTT) extrapure, 98% (Cleland’s Reagent) |
|---|---|
| Quantity/Pack Size | 1 Gms – 5 Gms – 25 Gms – 100 Gms |
| Form | Powder |
| Grade | Extrapure (98%) |
| Application | Molecular biology, protein reduction, biochemical assays |
Product Description
DL-Dithiothreitol (DTT), commonly known as Cleland’s Reagent, is a high-purity reducing agent essential for molecular biology and biochemical research. With a purity of 98%, this extrapure grade DTT ensures reliable performance in protein reduction, disulfide bond cleavage, and enzyme activation. Whether you’re working on protein purification, DNA extraction, or cell lysis, this lab-grade DTT powder delivers consistent results.
Researchers and students trust DTT for its ability to maintain reducing conditions in buffers, preventing oxidation of sensitive biomolecules. The extrapure quality guarantees minimal impurities, making it suitable for critical applications like PCR, Western blotting, and protein crystallization. When you buy DTT powder from eqipped, you get a product that meets stringent laboratory standards.
Looking for a dependable DTT Cleland’s reagent price? eqipped offers competitive rates without compromising on quality. Our DTT for molecular biology is packaged in airtight containers to preserve stability, ensuring you receive a fresh and effective reagent every time. Whether you need a small quantity for a single experiment or bulk supply for ongoing research, we’ve got you covered.
Need high-purity DTT for your lab? Order now and experience seamless delivery across India.
DTT works by reducing disulfide bonds, which is crucial for maintaining protein structure and function. Its water-soluble nature makes it easy to incorporate into various buffers, and its fast reaction rate ensures efficient reduction. For best results, store DTT powder in a cool, dry place and prepare fresh solutions as needed to avoid degradation.
From academic labs to advanced research facilities, DTT remains a staple in biochemical toolkits. Its versatility extends to applications like RNA isolation, where it helps prevent RNAse activity, and in cell culture, where it supports cell viability. When sourcing a DTT supplier, prioritize purity and consistency—qualities that eqipped guarantees with every batch.
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FAQs
What is DTT used for in molecular biology?
DTT is primarily used to reduce disulfide bonds in proteins, preventing oxidation and maintaining protein structure. It’s essential in techniques like SDS-PAGE, Western blotting, and enzyme assays.
How do I prepare a DTT solution for lab use?
Dissolve DTT powder in ultrapure water or buffer to the desired concentration (commonly 1 M). Always prepare fresh solutions, as DTT oxidizes over time.
What’s the difference between DTT and β-mercaptoethanol?
DTT is more stable and has a lower odor compared to β-mercaptoethanol. It also provides a stronger reducing environment, making it preferred for sensitive applications.
Can I use DTT in cell culture?
Yes, DTT is used in cell culture to maintain reducing conditions, but concentrations must be optimized to avoid toxicity. Typically, 1-10 mM is used.
Where can I buy laboratory-grade DTT in India?
You can buy extrapure DTT online from eqipped, a trusted supplier of high-quality lab chemicals. We offer multiple pack sizes for your convenience.