Description
Specifications Table
| Product Name | Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 Mixture) |
|---|---|
| Quantity/Pack Size | 50 liters |
| Form | Liquid |
| Grade | Cell Culture Grade |
| Application | Cell Culture |
Product Description
Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 Mixture) is a specialized cell culture medium designed for a wide range of cell culture applications. This medium is a 1:1 mixture of DMEM and Ham’s F-12, providing a balanced nutrient composition that supports the growth and maintenance of various cell types.
Why is DMEM F12 medium essential for cell culture?
DMEM F12 medium is widely used in research and clinical laboratories for its ability to support the growth of both adherent and suspension cells. Its unique formulation ensures optimal cell viability and proliferation, making it an indispensable tool for scientists and researchers.
Key Features of DMEM F12 Medium:
This medium is enriched with essential amino acids, vitamins, and inorganic salts, providing a comprehensive nutrient source for cells. It is also supplemented with glucose, sodium pyruvate, and other components to enhance cell growth and metabolism.
Applications of DMEM F12 Medium:
DMEM F12 medium is used in various applications, including stem cell research, primary cell culture, and the cultivation of specialized cell lines. Its versatility makes it a popular choice for researchers working in fields such as oncology, neurology, and developmental biology.
Storage and Handling:
Store DMEM F12 medium at 2-8°C. Avoid exposure to direct sunlight and extreme temperatures. For long-term storage, aliquot the medium into smaller volumes and store at -20°C. Thaw aliquots at room temperature or in a water bath before use.
Safety Note:
DMEM F12 medium is a non-hazardous substance. However, it is recommended to handle the medium with standard laboratory precautions. Avoid contact with skin and eyes. In case of accidental contact, rinse thoroughly with water.
FAQs
What is the shelf life of DMEM F12 medium?
The shelf life of DMEM F12 medium is typically 12 months from the date of manufacture when stored at 2-8°C. Always check the expiration date on the product label before use.
Can DMEM F12 medium be used for primary cell culture?
Yes, DMEM F12 medium is suitable for primary cell culture. Its balanced nutrient composition supports the growth and maintenance of primary cells from various tissues.
How should I store DMEM F12 medium?
Store DMEM F12 medium at 2-8°C. For long-term storage, aliquot the medium into smaller volumes and store at -20°C. Thaw aliquots at room temperature or in a water bath before use.
Is DMEM F12 medium suitable for stem cell research?
Yes, DMEM F12 medium is commonly used in stem cell research. Its nutrient-rich formulation supports the growth and differentiation of stem cells, making it an ideal choice for this application.
What are the key components of DMEM F12 medium?
DMEM F12 medium contains essential amino acids, vitamins, inorganic salts, glucose, and sodium pyruvate. These components provide a comprehensive nutrient source for cells, supporting their growth and metabolism.
