Description
Specifications Table
| Product Name | Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 Mixture) |
|---|---|
| Quantity/Pack Size | 5 Liters |
| Form | Liquid |
| Grade | Cell Culture Grade |
| Application | Cell Culture |
Product Description
Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 Mixture) is a highly versatile and widely used cell culture medium. This 1:1 mixture of Dulbecco’s Modified Eagle Medium (DMEM) and Ham’s F-12 nutrient mixture provides a balanced environment for the growth and maintenance of various cell types. It is particularly suitable for the culture of epithelial cells, fibroblasts, and other adherent cell lines.
This medium is designed to support the proliferation and differentiation of cells, making it an essential component in cell biology research, tissue engineering, and biopharmaceutical production. The DMEM/F12 mixture is formulated with a precise blend of amino acids, vitamins, inorganic salts, and other essential components to ensure optimal cell growth and viability.
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DMEM/F12 is a serum-free medium, making it ideal for applications where serum-free conditions are required. It is also suitable for use in combination with various growth factors and supplements to tailor the culture environment to specific experimental needs. The medium is available in a convenient 5-liter pack size, ensuring a steady supply for your laboratory needs.
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FAQs
What is the primary use of DMEM F12 medium?
The primary use of DMEM F12 medium is to support the growth and maintenance of various cell types in cell culture. It is particularly useful for epithelial cells, fibroblasts, and other adherent cell lines.
What are the advantages of using DMEM F12 medium?
DMEM F12 medium offers several advantages, including a balanced nutrient composition, versatility in supporting various cell types, and the ability to be used in serum-free conditions. It is also suitable for use with growth factors and supplements.
How should DMEM F12 medium be stored?
DMEM F12 medium should be stored at 2-8°C (36-46°F) and protected from light. It is recommended to store the medium in its original packaging and avoid repeated freeze-thaw cycles.
Can DMEM F12 medium be used for serum-free culture?
Yes, DMEM F12 medium can be used for serum-free culture. Its formulation allows for the omission of serum, making it suitable for applications where serum-free conditions are required.
What is the shelf life of DMEM F12 medium?
The shelf life of DMEM F12 medium is typically 12 months from the date of manufacture when stored properly at 2-8°C (36-46°F). Always check the expiration date on the packaging before use.
