Description
Specifications Table
Product Name – EPPS, 99%, for biochemistry
Quantity/Pack Size – 100GR, 500GR
Form – Powder
Grade – Biochemistry
Application – Buffering agent in biochemical assays
Product Overview
EPPS (4-(2-Hydroxyethyl)piperazine-1-propanesulfonic acid) at 99% purity is a high-performance buffering agent specifically formulated for biochemistry applications. This zwitterionic buffer maintains stable pH across a wide temperature range, making it ideal for sensitive biochemical reactions. Its excellent water solubility and minimal interference with biological systems ensure reliable results in protein studies, enzyme assays, and molecular biology protocols. The powder form allows for precise concentration adjustments, while the biochemistry-grade certification guarantees low impurity levels that could affect experimental outcomes. EPPS demonstrates superior performance compared to traditional buffers like HEPES or Tris, particularly in applications requiring pH stability between 7.3-8.7. The compound’s resistance to enzymatic degradation and metal ion chelation properties further enhance its utility in complex biochemical systems. This product undergoes rigorous quality control to meet international laboratory standards, ensuring consistent performance across different experimental setups.
FAQs
1. What is the effective pH range for EPPS buffer?
EPPS maintains optimal buffering capacity between pH 7.3 and 8.7, making it particularly useful for physiological studies and enzyme reactions that require near-neutral pH conditions.
2. How does EPPS compare to HEPES buffer in biochemical applications?
EPPS offers similar pKa to HEPES but with better temperature stability and reduced interference in UV spectroscopy, making it preferable for applications requiring precise optical measurements.
3. What is the recommended storage temperature for EPPS powder?
Store EPPS powder at room temperature (15-25°C) in a tightly sealed container to prevent moisture absorption that could affect its buffering capacity.
4. Can EPPS be used in cell culture applications?
While primarily designed for biochemical assays, EPPS can be used in cell culture at appropriate concentrations, though specialized cell culture buffers may be preferred for long-term applications.
5. What is the typical working concentration range for EPPS in biochemical assays?
EPPS is commonly used at concentrations between 10-50 mM, though optimal concentration depends on specific assay requirements and desired buffering capacity.
