Description
Specifications Table
| Product Name | Fluorescein Sodium Salt (C.I. No. 45350) For Cell Culture, Endotoxin (Bet) 0.05eu/Mg | 98131 | SRL Chem |
|---|---|
| Pack Size | 100 Gms |
| Form | Powder |
| Grade | For Cell Culture |
| Application | Fluorescence microscopy, flow cytometry, cell viability assays |
Product Description
Fluorescein Sodium Salt is a versatile fluorescent dye widely used in biological research, particularly in cell culture applications. This high-purity grade, with an endotoxin level of ≤0.05 EU/mg, ensures minimal interference in sensitive experiments. Whether you’re conducting fluorescence microscopy, flow cytometry, or cell viability assays, this product delivers consistent and reliable results.
Researchers and educators trust Fluorescein Sodium Salt for its bright green fluorescence under UV or blue light, making it ideal for labeling cells, proteins, and other biomolecules. Its water-soluble nature allows for easy preparation of working solutions, while its stability ensures long-term usability in your lab.
When you buy Fluorescein Sodium Salt online from eqipped, you get a product that meets stringent quality standards, backed by SRL Chem’s reputation for excellence. Perfect for academic labs, research institutions, and biotech companies, this dye is a staple in any fluorescence-based workflow.
Key Applications
Fluorescein Sodium Salt is commonly used in:
- Cell viability and proliferation assays
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence staining
- Tracer studies in microfluidics
- pH-sensitive fluorescence experiments
Its broad excitation and emission spectra make it compatible with most standard fluorescence microscopes and flow cytometers, ensuring seamless integration into your existing protocols.
Why This Product Stands Out
Not all Fluorescein Sodium Salt products are created equal. This variant is specifically tested for low endotoxin levels, making it safer for cell culture applications where contamination can compromise results. The high purity (C.I. No. 45350) guarantees minimal background noise, allowing for clearer fluorescence signals.
Additionally, the 100g pack size offers excellent value for labs with frequent usage, reducing the need for constant reordering. Whether you’re a student setting up your first fluorescence experiment or a seasoned researcher optimizing a protocol, this product provides the consistency you need.
Safety and Handling
While Fluorescein Sodium Salt is generally safe for laboratory use, it’s important to handle it with standard precautions. Wear appropriate personal protective equipment (PPE), such as gloves and safety goggles, when preparing solutions. Store the product in a cool, dry place away from direct sunlight to maintain its stability.
For disposal, follow your institution’s guidelines for chemical waste to ensure environmental safety.
FAQs
What is Fluorescein Sodium Salt used for in cell culture?
It is primarily used as a fluorescent tracer or label in cell viability assays, immunofluorescence staining, and flow cytometry due to its bright green fluorescence.
How do I prepare a working solution of Fluorescein Sodium Salt?
Dissolve the powder in distilled water or a suitable buffer to the desired concentration, typically ranging from 0.1% to 1% (w/v), depending on the application.
Is this product suitable for live-cell imaging?
Yes, its low endotoxin level (≤0.05 EU/mg) makes it safe for live-cell applications without causing significant cytotoxicity.
What is the shelf life of Fluorescein Sodium Salt?
When stored properly in a cool, dry place, the product remains stable for up to 2 years. Always check the expiration date on the packaging.
Can I use this for pH-sensitive experiments?
Yes, Fluorescein Sodium Salt is pH-sensitive, with fluorescence intensity varying across different pH ranges, making it useful for pH monitoring in cellular environments.
